{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:29:40Z","timestamp":1790738980943,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T00:00:00Z","timestamp":1426204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Renal toxicity is a hallmark of uranium exposure, with uranium accumulating specifically in the S3 segment of the proximal tubules causing tubular damage. As the distribution, concentration and dynamics of accumulated uranium at the cellular level is not well understood, here, we report on high\u2010resolution quantitative <jats:italic>in situ<\/jats:italic> measurements by high\u2010energy synchrotron radiation X\u2010ray fluorescence analysis in renal sections from a rat model of uranium\u2010induced acute renal toxicity. One day after subcutaneous administration of uranium acetate to male Wistar rats at a dose of 0.5 mg uranium kg<jats:sup>\u20131<\/jats:sup> body weight, uranium concentration in the S3 segment of the proximal tubules was 64.9 \u00b1 18.2 \u00b5g g<jats:sup>\u20131<\/jats:sup>, sevenfold higher than the mean renal uranium concentration (9.7 \u00b1 2.4 \u00b5g g<jats:sup>\u20131<\/jats:sup>). Uranium distributed into the epithelium of the S3 segment of the proximal tubules and highly concentrated uranium (50\u2010fold above mean renal concentration) in micro\u2010regions was found near the nuclei. These uranium levels were maintained up to 8 days post\u2010administration, despite more rapid reductions in mean renal concentration. Two weeks after uranium administration, damaged areas were filled with regenerating tubules and morphological signs of tissue recovery, but areas of high uranium concentration (100\u2010fold above mean renal concentration) were still found in the epithelium of regenerating tubules. These data indicate that site\u2010specific accumulation of uranium in micro\u2010regions of the S3 segment of the proximal tubules and retention of uranium in concentrated areas during recovery are characteristics of uranium behavior in the kidney. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3126","type":"journal-article","created":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T13:14:15Z","timestamp":1426252455000},"page":"1594-1600","source":"Crossref","is-referenced-by-count":48,"title":["Cellular localization of uranium in the renal proximal tubules during acute renal uranium toxicity"],"prefix":"10.1002","volume":"35","author":[{"given":"Shino","family":"Homma\u2010Takeda","sequence":"first","affiliation":[{"name":"Research Center for Radiation Protection,Research, Development and Support Center National Institute of Radiological Sciences  Inage\u2010ku Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Kitahara","sequence":"additional","affiliation":[{"name":"Graduate School of Science Chiba University  Yayoi\u2010cho Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Research Center for Radiation Protection,Research, Development and Support Center National Institute of Radiological Sciences  Inage\u2010ku Chiba Japan"},{"name":"Graduate School of Agriculture and life Sciences University of Tokyo  Hongo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin J.","family":"Blyth","sequence":"additional","affiliation":[{"name":"Research Center for Radiation Protection,Research, Development and Support Center National Institute of Radiological Sciences  Inage\u2010ku Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyoshi","family":"Suya","sequence":"additional","affiliation":[{"name":"Research, Development and Support Center National Institute of Radiological Sciences  Inage\u2010ku Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruaki","family":"Konishi","sequence":"additional","affiliation":[{"name":"Research, Development and Support Center National Institute of Radiological Sciences  Inage\u2010ku Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Terada","sequence":"additional","affiliation":[{"name":"Japan Synchrotron Radiation Research Institute  Mikazuki Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiya","family":"Shimada","sequence":"additional","affiliation":[{"name":"Research Center for Radiation Protection,Research, Development and Support Center National Institute of Radiological Sciences  Inage\u2010ku Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfi035"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02892833"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx600347k"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-008-7312-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2870"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000080263"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004032-198909000-00001"},{"key":"e_1_2_7_9_1","first-page":"718","article-title":"Complexation of U(VI) with highly phosphorylated protein, phosvitin A vibrational spectroscopic approach","volume":"104","author":"Li B","year":"2010","journal-title":"J. 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