{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:35:31Z","timestamp":1789929331939,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,3,29]],"date-time":"2015-03-29T00:00:00Z","timestamp":1427587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To develop a testing strategy incorporating the human cell line activation test (h\u2010CLAT), direct peptide reactivity assay (DPRA) and DEREK, we created an expanded data set of 139 chemicals (102 sensitizers and 37 non\u2010sensitizers) by combining the existing data set of 101 chemicals through the collaborative projects of Japan Cosmetic Industry Association. Of the additional 38 chemicals, 15 chemicals with relatively low water solubility (log Kow &gt; 3.5) were selected to clarify the limitation of testing strategies regarding the lipophilic chemicals. Predictivities of the h\u2010CLAT, DPRA and DEREK, and the combinations thereof were evaluated by comparison to results of the local lymph node assay. When evaluating 139 chemicals using combinations of three methods based on integrated testing strategy (ITS) concept (ITS\u2010based test battery) and a sequential testing strategy (STS) weighing the predictive performance of the h\u2010CLAT and DPRA, overall similar predictivities were found as before on the 101 chemical data set. An analysis of false negative chemicals suggested a major limitation of our strategies was the testing of low water\u2010soluble chemicals. When excluded the negative results for chemicals with log Kow &gt; 3.5, the sensitivity and accuracy of ITS improved to 97% (91 of 94 chemicals) and 89% (114 of 128). Likewise, the sensitivity and accuracy of STS to 98% (92 of 94) and 85% (111 of 129). Moreover, the ITS and STS also showed good correlation with local lymph node assay on three potency classifications, yielding accuracies of 74% (ITS) and 73% (STS). Thus, the inclusion of log Kow in analysis could give both strategies a higher predictive performance. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3127","type":"journal-article","created":{"date-parts":[[2015,3,29]],"date-time":"2015-03-29T23:00:17Z","timestamp":1427670017000},"page":"1318-1332","source":"Crossref","is-referenced-by-count":102,"title":["Test battery with the human cell line activation test, direct peptide reactivity assay and DEREK based on a 139 chemical data set for predicting skin sensitizing potential and potency of chemicals"],"prefix":"10.1002","volume":"35","author":[{"given":"Osamu","family":"Takenouchi","sequence":"first","affiliation":[{"name":"Kao Corporation  Ichikai\u2010Machi Haga\u2010Gun, Tochigi Japan"},{"name":"Working Group for <i>In Vitro<\/i> Skin Sensitization Evaluation in Japan Cosmetic Industry Association"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiho","family":"Fukui","sequence":"additional","affiliation":[{"name":"Kanebo Cosmetics Inc.  Odawara Kanagawa Japan"},{"name":"Working Group for <i>In Vitro<\/i> Skin Sensitization Evaluation in Japan Cosmetic Industry Association"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Kanebo Cosmetics Inc.  Odawara Kanagawa Japan"},{"name":"Working Group for <i>In Vitro<\/i> Skin Sensitization Evaluation in Japan Cosmetic Industry Association"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kurotani","sequence":"additional","affiliation":[{"name":"KOSE Corporation  Itabashi\u2010ku Tokyo Japan"},{"name":"Working Group for <i>In Vitro<\/i> Skin Sensitization Evaluation in Japan Cosmetic Industry Association"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyasu","family":"Imai","sequence":"additional","affiliation":[{"name":"KOSE Corporation  Itabashi\u2010ku Tokyo Japan"},{"name":"Working Group for <i>In Vitro<\/i> Skin Sensitization Evaluation in Japan Cosmetic Industry Association"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyuki","family":"Fujishiro","sequence":"additional","affiliation":[{"name":"Cosmos Technical Center Co., Ltd.  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