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In FETAX, a 48\u2009h continuous exposure is usually conducted when the <jats:italic>X<\/jats:italic>. <jats:italic>tropicalis<\/jats:italic> embryo is used as the test model. In the present study, we exposed <jats:italic>X<\/jats:italic>. <jats:italic>tropicalis<\/jats:italic> embryos to nine known teratogens for four separate 12\u2010h periods. The embryos showed great variations in response to nine tested compounds during different exposure periods. Based on the value of the score of malformations, the most sensitive 12\u2009h exposure periods of embryos were significantly distinguished for all the compounds with the exception of NiCl<jats:sub>2<\/jats:sub>. The embryos were the most sensitive to retinols (e.g. <jats:italic>all\u2010trans<\/jats:italic>\u2010retinoic acid and <jats:italic>9\u2010cis<\/jats:italic>\u2010retinoic acid) during 0\u201312\u2009h and to metal compounds (e.g. triphenlytin and CdCl<jats:sub>2<\/jats:sub>) during a 24 to 36\u2009h exposure period. In the further 3\u2009h exposure experiment, the most sensitive period could only be determined for one of three tested compounds. Based on the present results, we proposed an assay to determine a 12\u2009h sensitive window of embryos to chemical exposure using <jats:italic>Xenopus tropicalis<\/jats:italic>. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3200","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T17:24:58Z","timestamp":1436894698000},"page":"685-691","source":"Crossref","is-referenced-by-count":5,"title":["An assay to determine the sensitive window of embryos to chemical exposure using <i>Xenopus tropicalis<\/i>"],"prefix":"10.1002","volume":"36","author":[{"given":"Lingling","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Estuarine and Costal Research East China Normal University  Shanghai 200062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijiao","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Estuarine and Costal Research East China Normal University  Shanghai 200062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingang","family":"Xue","sequence":"additional","affiliation":[{"name":"Changzhou Environmental Monitoring Center  Jiangsu 213001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingmin","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Estuarine and Costal Research East China Normal University  Shanghai 200062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huahong","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Estuarine and Costal Research East China Normal University  Shanghai 200062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es049765n"},{"key":"e_1_2_8_3_1","first-page":"447","volume-title":"ASTM Standards on Biological Effects and 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