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Previous\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies showed that the total amount of compound, regardless of properties, penetrating over a 96\u2009h period could be predicted by the amount present in SC 30\u2009min after application by a linear relationship. Validating this linear relationship through\n                    <jats:italic>in vitro<\/jats:italic>\n                    study would facilitate testing of transdermal drug delivery platforms. We aimed to determine\n                    <jats:italic>in vitro<\/jats:italic>\n                    penetration behavior across SC of humans by determining the relationship between quantity present in SC reservoir 30\u2009min after application with 24\u2009h skin absorption and penetration. In this study, use of the SC reservoir effect to predict absorption and penetration of topical compounds is reaffirmed with\n                    <jats:italic>in vitro<\/jats:italic>\n                    models involving human skin. These results indicate the amount in short\u2010term (30\u2009min) SC reservoir predict long\u2010term (24\u2009h) skin absorption and penetration, as characterized by statistically significant linear relationships determined via regression. This may be explained by the fact that SC is a rate\u2010limiting barrier to percutaneous drug transport. After molecules diffuse through SC barrier, passage into deeper dermal layers and systemic uptake occur relatively quickly. These results enable one to measure quantity in SC reservoir shortly after topical application as a proxy for absorption and penetration over longer periods. With respect to drug development and risk assessment of toxic substances, this may simplify assays attempting to quantitate penetration capacity. Further investigation with a larger range of compounds is needed to clarify the observations recorded here. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/jat.3262","type":"journal-article","created":{"date-parts":[[2015,11,27]],"date-time":"2015-11-27T22:26:39Z","timestamp":1448663199000},"page":"1003-1010","source":"Crossref","is-referenced-by-count":14,"title":["Stratum corneum reservoir as a predictive method for\n                    <i>in vitro<\/i>\n                    percutaneous absorption"],"prefix":"10.1002","volume":"36","author":[{"given":"Farhaan","family":"Hafeez","sequence":"first","affiliation":[{"name":"University of California  San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audris","family":"Chiang","sequence":"additional","affiliation":[{"name":"University of California  San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Hui","sequence":"additional","affiliation":[{"name":"University of California  San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"University of California  San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faraz","family":"Kamili","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard I.","family":"Maibach","sequence":"additional","affiliation":[{"name":"University of California  San Francisco CA 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