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Thus, the aim of our study was to establish an <jats:italic>in vitro<\/jats:italic> assay as a screening tool for detecting skin sensitizers using the human keratinocyte cell line, HaCaT. HaCaT cells were exposed to 16 relevant skin sensitizers and 6 skin non\u2010sensitizers. The highest dose used was the dose causing 75% cell viability (CV75) that we determined by an MTT [3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide] assay. The levels of extracellular production of interleukin\u20101\u03b1 (IL\u20101\u03b1) and IL\u20106 were measured. The sensitivity of IL\u20101\u03b1 was 63%, specificity was 83% and accuracy was 68%. In the case of IL\u20106, sensitivity: 69%, specificity: 83% and accuracy: 73%. Thus, this study suggests that measuring extracellular production of pro\u2010inflammatory cytokines IL\u20101\u03b1 and IL\u20106 by human HaCaT cells may potentially classify skin sensitizers from non\u2010sensitizers. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3274","type":"journal-article","created":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T06:00:45Z","timestamp":1450764045000},"page":"1129-1136","source":"Crossref","is-referenced-by-count":24,"title":["Discrimination of skin sensitizers from non\u2010sensitizers by interleukin\u20101\u03b1 and interleukin\u20106 production on cultured human keratinocytes"],"prefix":"10.1002","volume":"36","author":[{"given":"Daun","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, and Institute of Endemic Disease College of Medicine, Seoul National University  Seoul 110\u2010799 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong\u2010Hwan","family":"Che","sequence":"additional","affiliation":[{"name":"Biomedical Research Institute Seoul National University Hospital  Seoul 110\u2010744 Republic of 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