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The assay is employed as a higher tiered approach to evaluate effects of chronic chemical exposure throughout multiple life stages in a model amphibian species, <jats:italic>Xenopus laevis<\/jats:italic>. To evaluate the utility of the initial LAGDA design, the assay was performed using a mixed mode of action endocrine disrupting chemical, benzophenone\u20102 (BP\u20102). <jats:italic>X. laevis<\/jats:italic> embryos were exposed in flow\u2010through conditions to 0, 1.5, 3.0 or 6.0 mg l<jats:sup>\u20131<\/jats:sup> BP\u20102 until 2 months post\u2010metamorphosis. Overt toxicity was evident throughout the exposure period in the 6.0 mg l<jats:sup>\u20131<\/jats:sup> treatment due to elevated mortality rates and observed liver and kidney pathologies. Concentration\u2010dependent increases in severity of thyroid follicular cell hypertrophy and hyperplasia occurred in larval tadpoles indicating BP\u20102\u2010induced impacts on the thyroid axis. Additionally, gonads were impacted in all treatments with some genetic males showing both testis and ovary tissues (1.5 mg l<jats:sup>\u20131<\/jats:sup>) and 100% of the genetic males in the 3.0 and 6.0 mg l<jats:sup>\u22121<\/jats:sup> treatments experiencing complete male\u2010to\u2010female sex reversal. Concentration\u2010dependent vitellogenin induction occurred in both genders with associated accumulations of protein in the livers, kidneys and gonads, which was likely vitellogenin and other estrogen\u2010responsive yolk proteins. This is the first study that demonstrates the endocrine effects of this mixed mode of action chemical in an amphibian species and demonstrates the utility of the LAGDA design for supporting chemical risk assessment. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3336","type":"journal-article","created":{"date-parts":[[2016,5,30]],"date-time":"2016-05-30T22:22:47Z","timestamp":1464646967000},"page":"1651-1661","source":"Crossref","is-referenced-by-count":31,"title":["Development of the Larval Amphibian Growth and Development Assay: Effects of benzophenone\u20102 exposure in <i>Xenopus laevis<\/i> from embryo to juvenile"],"prefix":"10.1002","volume":"36","author":[{"given":"Jonathan T.","family":"Haselman","sequence":"first","affiliation":[{"name":"US EPA ORD\/NHEERL\/Mid\u2010Continent Ecology Division  6201 Congdon Blvd Duluth MN 55804 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Institute of Environmental Ecology IDEA Consultants Inc.  1334\u20105 Riemon, Yaizu Shizuoka 421\u20100212 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