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However, the effect of INH on fetal growth has not yet been elucidated, and the mechanism of INH\u2010induced developmental toxicity is still unknown. In the present study, we employed zebrafish embryos and larvae to investigate the developmental toxicity of INH. The survival rates of the embryos and larvae as well as the hatching rates of embryos were significantly reduced. Morphological abnormalities, including spinal curvature, yolk retention, swimming bladder absence, tail bending and shorter body lengths were induced by INH. Histopathological analysis showed loose cell\u2010to\u2010cell contacts and large vacuoles in the larval hepatocytes. Thin intestinal walls, frayed gut villi and widespread cell lysis were observed in the intestines of the larvae in the higher concentration (8, 16\u2009m<jats:sc>m<\/jats:sc>) exposure groups. In addition, exposure to high doses (\u2265 6\u2009m<jats:sc>m<\/jats:sc>) of INH significantly reduced the locomotor capacity of the zebrafish larvae. INH significantly increased the levels of reactive oxygen species and malondialdehyde and decreased the superoxide dismutase activity in zebrafish larvae, which suggested that oxidative stress was induced and that the antioxidant capacity was inhibited. Superoxide dismutase 1 and liver fatty acid\u2010binding protein mRNA levels were significantly downregulated, while the GSTP2 and cytochrome P450 3A mRNA levels were significantly upregulated in the INH\u2010exposed zebrafish larvae. The overall results indicated that INH caused a dose\u2010 and time\u2010dependent increase in developmental toxicity and that oxidative stress played an important role in the developmental toxicity induced by INH in zebrafish larvae. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3432","type":"journal-article","created":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T00:56:56Z","timestamp":1485824216000},"page":"842-852","source":"Crossref","is-referenced-by-count":50,"title":["Oxidative stress\u2010mediated developmental toxicity induced by isoniazide in zebrafish embryos and larvae"],"prefix":"10.1002","volume":"37","author":[{"given":"Yu","family":"Zou","sequence":"first","affiliation":[{"name":"Key Laboratory of Natural Medicine and Immuno\u2010Engineering Henan University  Kaifeng 475004 Henan Province People's Republic of China"},{"name":"Biology Institute of Shandong Academy of Sciences  19 Keyuan Road, Lixia District Jinan 250014 Shandong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biology Institute of Shandong Academy of Sciences  19 Keyuan 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