{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:29:36Z","timestamp":1765546176784,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["H25\u2010KAGAKU\u2010IPPANN\u2010003"],"award-info":[{"award-number":["H25\u2010KAGAKU\u2010IPPANN\u2010003"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Correct perinatal oestrogen levels are critical for sexual differentiation. For example, perinatal exposure to oestrogen causes masculinization and defeminization of the brain in female rats and also induces delayed effects after maturation characterized by early onset of abnormal oestrus cycling. However, the mechanisms underlying the above effects of oestrogen remain to be fully determined. 17\u03b1\u2010ethinyloestradiol (EE), a common synthetic oestrogen widely used in oral contraceptives, binds specifically to oestrogen receptors. In this study, we demonstrated the effects of a single neonatal injection of high\u2010 or low\u2010dose EE on reproductive behaviours. Female rats within 24\u2009h after birth were subcutaneously injected with sesame oil, EE (0.02, 2\u2009mg kg<jats:sup>\u22121<\/jats:sup>) and 17\u03b2\u2010oestradiol (E<jats:sub>2<\/jats:sub>) (20\u2009mg kg<jats:sup>\u22121<\/jats:sup>). Between 11 and 15\u2009weeks of age, sexual behaviour was tested twice in a paced mating situation. Latency to enter, lordosis and soliciting behaviour were recorded. Both high\u2010dose EE\u2010 and E<jats:sub>2<\/jats:sub>\u2010treated females showed a significantly lower lordosis quotient, decreased soliciting behaviours, increased rejection and fighting numbers. Accessibility to males was also delayed by neonatal E<jats:sub>2<\/jats:sub> exposure, although it was shortened by high\u2010dose EE exposure. In contrast, low\u2010dose EE\u2010treated females did not exhibit impaired sexual behaviour. These results suggest that single neonatal exposure to a high dose of EE or E<jats:sub>2<\/jats:sub> disturbs the normal development of the female brain, resulting in impaired sexual behaviours in a female\u2010paced mating situation. Besides, the differences noted between high\u2010dose EE\u2010 and E<jats:sub>2<\/jats:sub>\u2010treated females might be caused by different affinities of the oestrogen receptors, metabolic rates or mechanisms of action. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3449","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T04:46:43Z","timestamp":1486529203000},"page":"996-1003","source":"Crossref","is-referenced-by-count":5,"title":["Effects of neonatal 17\u03b1\u2010ethinyloestradiol exposure on female\u2010paced mating behaviour in the rat"],"prefix":"10.1002","volume":"37","author":[{"given":"Chiaki","family":"Komine","sequence":"first","affiliation":[{"name":"Laboratory of Animal Behavior and Environmental Science School of Agriculture Meiji University  1\u20101\u20101 Higashimita, Tama\u2010ku Kawasaki Kanagawa 214\u20108571 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Behavior and Environmental Science School of Agriculture Meiji University  1\u20101\u20101 Higashimita, Tama\u2010ku Kawasaki Kanagawa 214\u20108571 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences Teikyo University of Science  2\u20102\u20101 Senju\u2010Sakuragi, Adachi\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Horii","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Behavior and Environmental Science School of Agriculture Meiji University  1\u20101\u20101 Higashimita, Tama\u2010ku Kawasaki Kanagawa 214\u20108571 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Midori","family":"Yoshida","sequence":"additional","affiliation":[{"name":"National Institute of Health Sciences  1\u201018\u20101 Kamiyoga, Setagaya\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3184-9483","authenticated-orcid":false,"given":"Maiko","family":"Kawaguchi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Behavior and Environmental Science School of Agriculture Meiji University  1\u20101\u20101 Higashimita, Tama\u2010ku Kawasaki Kanagawa 214\u20108571 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2004.04.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2009.03.011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.54.1.105"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2013.12.009"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2006.02.004"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018\u2010506X(88)90005\u20100"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001289900925"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21490"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165\u20103806(01)00205\u2010X"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2004.04.006"},{"key":"e_1_2_7_12_1","first-page":"1187","article-title":"Half\u2010lives in plasma and bioavailability of ethinylestradiol in laboratory animals","volume":"36","author":"D\u00fcsterberg B","year":"1986","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735\u20107044.99.1.151"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2016.06.016"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.02110s3409"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxic\/kfu077"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2014.05.003"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009\u20101022"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2008.08.009"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118891094"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2170\/jjphysiol.RP001105"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.17096"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6163211"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2014.00242"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE\u201007\u20100014"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2009.06.012"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.12.6358"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.12.6357"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12887"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166\u20104328(99)00087\u2010X"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169\u2010328X(00)00135\u20102"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/34.2.288"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfp266"},{"key":"e_1_2_7_34_1","first-page":"155","volume-title":"Neural Control of Reproduction: Physiology and Behavior","author":"Sakuma Y","year":"1997"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devbrainres.2004.06.013"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146136"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2006.12.004"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2013.05.005"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.39.775"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010016\u20101858\u20104"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/hbeh.1997.1412"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2015.10.040"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.3449","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3449","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:59:15Z","timestamp":1760525955000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.3449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,8]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1002\/jat.3449"],"URL":"https:\/\/doi.org\/10.1002\/jat.3449","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"type":"print","value":"0260-437X"},{"type":"electronic","value":"1099-1263"}],"subject":[],"published":{"date-parts":[[2017,2,8]]}}}