{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:41:04Z","timestamp":1760575264772,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T00:00:00Z","timestamp":1506556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundations of China","doi-asserted-by":"crossref","award":["81273098","81673140"],"award-info":[{"award-number":["81273098","81673140"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Scientific Research Foundation of Guangdong Province","award":["A2017193","A2016557","A2016137","B2014070","B2014017","A2012068"],"award-info":[{"award-number":["A2017193","A2016557","A2016137","B2014070","B2014017","A2012068"]}]},{"name":"Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment","award":["2012A061400007"],"award-info":[{"award-number":["2012A061400007"]}]},{"name":"Young teacher training Program of Sun Yat-sen University","award":["15ykpy06"],"award-info":[{"award-number":["15ykpy06"]}]},{"name":"Science and Technology Planning Project of Guangzhou City","award":["201707010078","201707010405"],"award-info":[{"award-number":["201707010078","201707010405"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The identification of aberrant microRNA (miRNA) expression during chemical\u2010induced hepatic dysfunction will lead to a better understanding of the substantial role of miRNAs in liver diseases. 1,2\u2010Dichloroethane (1,2\u2010DCE), a chlorinated organic toxicant, can lead to hepatic abnormalities in occupationally exposed populations. To explore whether aberrant miRNA expression is involved in liver abnormalities mediated by 1,2\u2010DCE exposure, we examined alterations in miRNA expression patterns in the livers of NIH Swiss mice after dynamic inhalation exposure to 350 or 700 mg m<jats:sup>\u20133<\/jats:sup> 1,2\u2010DCE for 28 days. Using a microarray chip, we discovered that only mmumiR\u2010451a was significantly upregulated in the liver tissue of mice exposed to 700 mg m<jats:sup>\u20133<\/jats:sup> 1,2\u2010DCE; this finding was validated by quantitative real\u2010time polymerase chain reaction. In vitro study revealed that it was metabolite 2\u2010chloroacetic acid, not 1,2\u2010DCE that resulted in the upregulation of mmu\u2010miR\u2010451a in the mouse AML12 cell line. Furthermore, our data showed that the upregulation of mmu\u2010miR\u2010451a induced by 2\u2010chloroacetic acid could suppress the expression of glycerol kinase and lead to the inhibition of glycerol gluconeogenesis in mouse liver tissue and AML12 cells. These observations provide evidence that hepatic mmu\u2010miR\u2010451a responds to 1,2\u2010DCE exposure and might induce glucose metabolism disorders by suppressing the glycerol gluconeogenesis process.<\/jats:p>","DOI":"10.1002\/jat.3526","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T12:01:34Z","timestamp":1506600094000},"page":"292-303","source":"Crossref","is-referenced-by-count":9,"title":["Aberrant expression of miR\u2010451a contributes to 1,2\u2010dichloroethane\u2010induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1325-9105","authenticated-orcid":false,"given":"Ni","family":"Zeng","sequence":"first","affiliation":[{"name":"Faculty of Preventive Medicine, A Key Laboratory of Guangzhou Environmental Pollution and Risk Assessment, School of Public Health Sun Yat\u2010sen University  Guangzhou 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