{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:40:07Z","timestamp":1760575207087,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T00:00:00Z","timestamp":1514246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000202","name":"U.S. Fish and Wildlife Service","doi-asserted-by":"publisher","award":["Great Lakes Restoration Initiative"],"award-info":[{"award-number":["Great Lakes Restoration Initiative"]}],"id":[{"id":"10.13039\/100000202","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Contaminants of emerging concern (CECs) are ubiquitous in aquatic environments with well\u2010established endocrine\u2010disrupting effects. A data matrix of 559 water samples was queried to identify two commonly occurring CECs mixtures in Great Lakes tributaries. One mixture consisted of eight agricultural CECs (AG), while another contained 11 urban CECs (UB). The known estrogenic compounds bisphenol A, estrone and nonylphenol were present in both mixtures. According to the EPA Tox21 in ToxCast database, AG and UB mixture at an environmentally relevant concentration were estimated to account for 6.5% and 3.4% estrogenicity of the model endocrine disruptor estradiol\u201017\u03b2, respectively. Two isoforms of the estrogen receptor (Esr1 and \u20102, former Er\u03b1 and Er\u03b2) cloned from fathead minnow, bluegill sunfish, American alligator and human, responded differently to AG and UB mixtures. Human and bluegill Esr1 were the most sensitive to AG and UB mixtures, respectively. Fathead minnow Esr1 and Esr2b were the least sensitive to 10\u00d7 AG and UB in estrogen dose equivalents, respectively. Even at environmentally documented concentrations, UB significantly activated bluegill Esr1. Moreover, 100\u00d7 concentrated UB hyperstimulated fathead minnow Esr1 beyond the maximum induction of estradiol\u201017\u03b2. These results indicate that efficacious receptors and species differ in their response to CEC mixtures. Furthermore, estrogenicity may be present in some CECs not previously considered estrogenic, or, alternatively, estrogenicity of a mixture may be enhanced through chemical interactions. Our study highlights the need for further studies of CECs utilizing a variety of receptors cloned from diverse species.<\/jats:p>","DOI":"10.1002\/jat.3577","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T02:12:38Z","timestamp":1514254358000},"page":"705-713","source":"Crossref","is-referenced-by-count":5,"title":["Divergent responsiveness of two isoforms of the estrogen receptor to mixtures of contaminants of emerging concern in four vertebrates"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6184-3616","authenticated-orcid":false,"given":"Satomi","family":"Kohno","sequence":"first","affiliation":[{"name":"Aquatic Toxicology Laboratory St. Cloud State University  St. Cloud MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinao","family":"Katsu","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Hokkaido University  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Cipoletti","sequence":"additional","affiliation":[{"name":"Aquatic Toxicology Laboratory St. Cloud State University  St. Cloud MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina C.","family":"Wang","sequence":"additional","affiliation":[{"name":"Aquatic Toxicology Laboratory St. Cloud State University  St. Cloud MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary G.","family":"Jorgenson","sequence":"additional","affiliation":[{"name":"Aquatic Toxicology Laboratory St. Cloud State University  St. Cloud MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Miyagawa","sequence":"additional","affiliation":[{"name":"Institute of Advanced Medicine Wakayama Medical University  Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heiko L.","family":"Schoenfuss","sequence":"additional","affiliation":[{"name":"Aquatic Toxicology Laboratory St. Cloud State University  St. Cloud MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,26]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Ankley G. 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