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Chronic exposure to Cd has been correlated to lung disease development including cancers. Although many studies have been conducted to investigate the proteome response of cells challenged with Cd, the epiproteomic responses (i.e., global histone post\u2010translational modifications [PTMs]), particularly in human lung cells, are largely unexplored. Here, we provide an epiproteome profiling of human bronchial epithelial cells (BEAS\u20102B) chronically treated with cadmium chloride (CdCl<jats:sub>2<\/jats:sub>), with the aim of identifying global epiproteomic signatures in response to Cd epigenotoxicity. Total histone proteins from Cd\u2010treated and untreated BEAS\u20102B cells were isolated and subject to quantitative histone PTM\u2013enzyme\u2010linked immunosorbent assay using 18 histone PTM antibodies. Our results unveiled that chronic Cd treatment led to the marked downregulation of H3K4me2 and H3K36me3 and upregulation of H3K9acS10ph, H4K5ac, H4K8ac and H4K12ac PTM marks. Cd\u2010treated cells exhibit transformed cell properties as evidenced by enhanced cell migration and the ability of anchorage\u2010independent growth on soft agar. Notably, treatment of Cd\u2010transformed cells with C646, a potent histone acetyltransferase inhibitor, suppressed the expression of mesenchymal marker genes and cell migration ability of these cells. Taken together, our studies provide for the first time the global epiproteomic interrogation of chronic Cd\u2010exposed human lung cells. The identified aberrant histone PTM alterations associated with Cd\u2010induced epigenotoxicity likely account for the epithelial\u2212mesenchymal transition and neoplastic survival of these cells.<\/jats:p>","DOI":"10.1002\/jat.3597","type":"journal-article","created":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T03:33:53Z","timestamp":1518147233000},"page":"888-895","source":"Crossref","is-referenced-by-count":25,"title":["Epiproteome profiling of cadmium\u2010transformed human bronchial epithelial cells by quantitative histone post\u2010translational modification\u2013enzyme\u2010linked immunosorbent assay"],"prefix":"10.1002","volume":"38","author":[{"given":"Zhan\u2010Ling","family":"Liang","sequence":"first","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan\u2010Dan","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"},{"name":"Department of Pathology and Medical Biology University Medical Center Groningen, University of Groningen  Groningen The Netherlands"},{"name":"GRIAC Research Institute University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Yao","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei\u2010Yuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng Wee","family":"Tan","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6732-8903","authenticated-orcid":false,"given":"Machteld N.","family":"Hylkema","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University Medical Center Groningen, University of Groningen  Groningen The Netherlands"},{"name":"GRIAC Research Institute University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7416-2788","authenticated-orcid":false,"given":"Marianne G.","family":"Rots","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1124-0045","authenticated-orcid":false,"given":"Yan\u2010Ming","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics Shantou University Medical College  Shantou Guangdong 515041 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7146-7789","authenticated-orcid":false,"given":"Andy T. 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