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The aim of this study was to evaluate the effect of zinc (Zn) supplementation (30 or 60\u00a0mg\u00a0l<jats:sup>\u20131<\/jats:sup>) under moderate and relatively high exposure to Cd (5 and 50\u00a0mg\u00a0l<jats:sup>\u20131<\/jats:sup>) on collagen in the rat tibia proximal epiphysis and diaphysis (regions abundant in trabecular and cortical bone, respectively). Significant decrease in collagen type I biosynthesis was found in both regions of the tibia in Cd\u2010treated rats, whereas the supplementation with Zn provided significant protection against this effect. Western blot confirmed the presence of the major type I collagen in the tibia epiphysis and diaphysis, but collagen type II was revealed only in the epiphysis. Acetic acid\u2010 and pepsin\u2010soluble collagen concentration in the tibia epiphysis and diaphysis was significantly increased due to the exposure to Cd, whereas the supplementation with Zn protected, partially or totally, from these effects, depending on the used concentration. The supplementation with Zn also provided protection from unfavorable Cd impact on the maturation of the bone collagen, as the ratio of cross\u2010links to monomers was higher compared to the Cd\u2010treated group. This report confirms our previous findings on the preventive action of Zn against harmful effects of Cd on bone, but additionally, and to the best of our knowledge for the first time, explains the possible mechanism of the beneficial influence of this bioelement.<\/jats:p>","DOI":"10.1002\/jat.3608","type":"journal-article","created":{"date-parts":[[2018,3,5]],"date-time":"2018-03-05T21:58:45Z","timestamp":1520287125000},"page":"996-1007","source":"Crossref","is-referenced-by-count":16,"title":["Beneficial impact of zinc supplementation on the collagen in the bone tissue of cadmium\u2010exposed rats"],"prefix":"10.1002","volume":"38","author":[{"given":"Edyta","family":"Andrulewicz\u2010Botuli\u0144ska","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry Medical University of Bialystok  Kilinskiego 1 15\u2010230 Bialystok 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