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EpiSensA has exhibited an accuracy of 90% for 72 chemicals, including lipophilic chemicals and pre\u2212\/pro\u2010haptens, when compared with the results of the murine local lymph node assay. In this work, a ring study was performed by one lead and two naive laboratories to evaluate the transferability, as well as within\u2010 and between\u2010laboratory reproducibilities, of EpiSensA. Three non\u2010coded chemicals (two lipophilic sensitizers and one non\u2010sensitizer) were tested for the assessment of transferability and 10 coded chemicals (seven sensitizers and three non\u2010sensitizers, including four lipophilic chemicals) were tested for the assessment of reproducibility. In the transferability phase, the non\u2010coded chemicals (two sensitizers and one non\u2010sensitizer) were correctly classified at the two naive laboratories, indicating that the EpiSensA protocol was transferred successfully. For the within\u2010laboratory reproducibility, the data generated with three coded chemicals tested in three independent experiments in each laboratory gave consistent predictions within laboratories. For the between\u2010laboratory reproducibility, 9 of the 10 coded chemicals tested once in each laboratory provided consistent predictions among the three laboratories. These results suggested that EpiSensA has good transferability, as well as within\u2010 and between\u2010laboratory reproducibility.<\/jats:p>","DOI":"10.1002\/jat.3634","type":"journal-article","created":{"date-parts":[[2018,5,3]],"date-time":"2018-05-03T07:06:46Z","timestamp":1525331206000},"page":"1233-1243","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transferability and within\u2010 and between\u2010laboratory reproducibilities of EpiSensA for predicting skin sensitization potential in vitro: A ring study in three laboratories"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8778-1951","authenticated-orcid":false,"given":"Hideyuki","family":"Mizumachi","sequence":"first","affiliation":[{"name":"Kao Corporation, R&amp;D, Safety Science Research  2606 Akabane, Ichikai\u2010Machi, Haga\u2010Gun Tochigi 321\u20103497 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Sakuma","sequence":"additional","affiliation":[{"name":"KOS\u00c9 Corporation, Research Laboratories  1\u201018\u20104 Azusawa, Itabashi\u2010ku Tokyo 174\u20100051 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayu","family":"Ikezumi","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Center Hatano Research Institute  729\u20105 Ochiai Hadano\u2010shi Kanagawa 257\u20108523 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutoshi","family":"Saito","sequence":"additional","affiliation":[{"name":"Kao Corporation, R&amp;D, Safety Science Research  2606 Akabane, Ichikai\u2010Machi, Haga\u2010Gun Tochigi 321\u20103497 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Midori","family":"Takeyoshi","sequence":"additional","affiliation":[{"name":"KOS\u00c9 Corporation, Research Laboratories  1\u201018\u20104 Azusawa, Itabashi\u2010ku Tokyo 174\u20100051 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyasu","family":"Imai","sequence":"additional","affiliation":[{"name":"KOS\u00c9 Corporation, Research Laboratories  1\u201018\u20104 Azusawa, Itabashi\u2010ku Tokyo 174\u20100051 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Okutomi","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Center Hatano Research Institute  729\u20105 Ochiai Hadano\u2010shi Kanagawa 257\u20108523 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asami","family":"Umetsu","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Center Hatano Research Institute  729\u20105 Ochiai Hadano\u2010shi Kanagawa 257\u20108523 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Motohashi","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Center Hatano Research Institute  729\u20105 Ochiai Hadano\u2010shi Kanagawa 257\u20108523 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Center Hatano Research Institute  729\u20105 Ochiai Hadano\u2010shi Kanagawa 257\u20108523 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Miyazawa","sequence":"additional","affiliation":[{"name":"Kao Corporation, R&amp;D, Safety Science Research  2606 Akabane, Ichikai\u2010Machi, Haga\u2010Gun Tochigi 321\u20103497 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569527.2014.979425"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.00944.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2000.042006344.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.109439"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00272.2011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/026119290903700313"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2016.01.007"},{"key":"e_1_2_7_9_1","unstructured":"EC EURL ECVAM. 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