{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T22:07:35Z","timestamp":1771884455400,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T00:00:00Z","timestamp":1527033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Photoallergic dermatitis, caused by pharmaceuticals and other consumer products, is a very important issue in human health. However, S10 guidelines of the International Conference on Harmonization do not recommend the existing prediction methods for photoallergy because of their low predictability in human cases. We applied local lymph node assay (LLNA), a reliable, quantitative skin sensitization prediction test, to develop a new photoallergy prediction method. This method involves a three\u2010step approach: (1) ultraviolet (UV) absorption analysis; (2) determination of no observed adverse effect level for skin phototoxicity based on LLNA; and (3) photoallergy evaluation based on LLNA. Photoallergic potential of chemicals was evaluated by comparing lymph node cell proliferation among groups treated with chemicals with minimal effect levels of skin sensitization and skin phototoxicity under UV irradiation (UV+) or non\u2010UV irradiation (UV\u2212). A case showing significant difference (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) in lymph node cell proliferation rates between UV\u2212 and UV+ groups was considered positive for photoallergic reaction. After testing 13 chemicals, seven human photoallergens tested positive and the other six, with no evidence of causing photoallergic dermatitis or UV absorption, tested negative. Among these chemicals, both doxycycline hydrochloride and minocycline hydrochloride were tetracycline antibiotics with different photoallergic properties, and the new method clearly distinguished between the photoallergic properties of these chemicals. These findings suggested high predictability of our method; therefore, it is promising and effective in predicting human photoallergens.<\/jats:p>","DOI":"10.1002\/jat.3643","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T00:44:40Z","timestamp":1527122680000},"page":"1316-1322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["New approach to predict photoallergic potentials of chemicals based on murine local lymph node assay"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-9851","authenticated-orcid":false,"given":"Yosuke","family":"Maeda","sequence":"first","affiliation":[{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Saitama Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University  Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruka","family":"Hirosaki","sequence":"additional","affiliation":[{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Takeyoshi","sequence":"additional","affiliation":[{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2010.01713.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2009.01550.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000008"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(90)90110-9"},{"key":"e_1_2_6_6_1","first-page":"13","article-title":"Use of UVB and UVA to induce and elicit contact photoallergy in the mouse","volume":"7","author":"Gerberick G. F.","year":"1990","journal-title":"Photodermatology, Photoimmunology & Photomedicine"},{"key":"e_1_2_6_7_1","first-page":"291","volume-title":"The Biologic Effects of Ultraviolet Radiation","author":"Herber L. C.","year":"1969"},{"key":"e_1_2_6_8_1","unstructured":"ICH. (2013).Photosafety Evaluation of Pharmaceuticals. ICH S10 Guideline."},{"key":"e_1_2_6_9_1","first-page":"124","volume-title":"Photosensitive dermatoses","author":"Matsuo I.","year":"2002"},{"key":"e_1_2_6_10_1","unstructured":"Ministry of Education Culture Sports Science and Technology. (2005).Act on Welfare and Management of Animals http:\/\/www.japaneselawtranslation.go.jp\/law\/detail\/?vm=04&re=01&id=61&lvm=01(accessed on 17 August 2017)."},{"key":"e_1_2_6_11_1","unstructured":"Ministry of Education Culture Sports Science and Technology. (2006).Guidelines for Proper Conduct of Animal Experiments http:\/\/www.scj.go.jp\/ja\/info\/kohyo\/pdf\/kohyo\u201020\u2010k16\u20102e.pdf(accessed on 17 August 2017)."},{"key":"e_1_2_6_12_1","unstructured":"OECD. (2010).OECD Guideline for the Testing of Chemicals No. 429 Skin Sensitization: Local Lymph Node Assay. 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