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As an example of the known MOAs, there are two MOAs of thyroid toxicity: inhibition of thyroid hormone synthesis (\u201cdirect\u201d effect) and hypermetabolism of thyroid hormone by enzyme induction in liver (\u201cindirect\u201d effect). In the present study, global profiles of gene expression were analyzed in rats treated with chemicals acting directly on the thyroid (thyroid peroxidase inhibitors such as propylthiouracil and methimazole) and chemicals acting indirectly on the thyroid (hepatic enzyme inducers such as phenobarbital and pregnenolone\u201016\u03b1\u2010carbonitrile) using microarrays. Using a subtraction method between these two types of chemicals, we identified characteristic gene expression changes on the thyroid hormone synthesis pathway by direct\u2010acting chemicals. Based on the functions of these genes, alterations of their expression seem to indicate the results of thyroid peroxidase inhibition, and might be helpful in more accurate evaluation of MOAs for thyroid toxicity.<\/jats:p>","DOI":"10.1002\/jat.3693","type":"journal-article","created":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T22:44:44Z","timestamp":1532558684000},"page":"1529-1537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Specific alteration of gene expression profile in rats by treatment with thyroid toxicants that inhibit thyroid hormone synthesis"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6672-3565","authenticated-orcid":false,"given":"Ayako","family":"Ohara","sequence":"first","affiliation":[{"name":"Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd.  1\u201098, 3\u2010Chome, Kasugade\u2010Naka, Konohana\u2010ku Osaka 554\u20108558 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