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We previously reported that MeHg (5 mg Hg\/kg) induced severe neurobehavioral dysfunction in 4\u2010week\u2010old KK\u2010Ay mice, although it is difficult to evaluate quantitatively the neurobehavioral impairment in MeHg\u2010treated KK\u2010Ay mice because of their obesity. The aim of this study was to evaluate MeHg\u2010induced neurobehavioral dysfunction in KK\u2010Ay mice using the dynamic weight\u2010bearing test, which analyzes the animal's weight distribution between the four limbs. Male 12\u2010week\u2010old KK\u2010Ay mice were treated with MeHg (5 mg Hg\/kg) three times per week for 5\u00a0weeks. Body weight loss began after approximately 2\u00a0weeks of MeHg treatment, and decreased significantly at 4\u00a0weeks. Seven of the nine MeHg\u2010treated mice exhibited overt neurological symptoms such as ataxia and gait disturbance. The weight\u2010bearing load was lower for the forelimb than for the hindlimb at baseline and until 1\u00a0week after MeHg treatment was initiated. In weeks 2\u20104, the dynamic weight\u2010bearing loads on the forelimb and hindlimb were similar. The load on the forelimb exceeded the load on the hindlimb after 5\u00a0weeks of treatment. This finding indicates that the dynamic weight\u2010bearing test is useful for semi\u2010quantitative evaluation of neurobehavioral impairment in MeHg\u2010treated rodents, and is less stressful for the animals. Infiltration of CD204\u2010positive macrophages was observed in the sciatic nerve of MeHg\u2010treated mice, suggesting that CD204 can serve as a useful marker of tissue injury in peripheral nerves and a possible target in regenerating peripheral nerves and controlling neuropathies.<\/jats:p>","DOI":"10.1002\/jat.3710","type":"journal-article","created":{"date-parts":[[2018,9,2]],"date-time":"2018-09-02T22:40:22Z","timestamp":1535928022000},"page":"221-230","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of neurobehavioral impairment in methylmercury\u2010treated KK\u2010Ay mice by dynamic weight\u2010bearing test"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4301-3827","authenticated-orcid":false,"given":"Megumi","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Integrated Physiology Section, Department of Basic Medical Science National Institute for Minamata Disease  4058\u201018 Hama, Minamata Kumamoto 867\u20100008 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eriko","family":"Motomura","sequence":"additional","affiliation":[{"name":"Integrated Physiology Section, Department of Basic Medical Science National Institute for Minamata Disease  4058\u201018 Hama, Minamata Kumamoto 867\u20100008 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Yanagisawa","sequence":"additional","affiliation":[{"name":"Center for Health and Environmental Risk Research National Institute for Environmental Studies  16\u20102 Onogawa, Tsukuba Ibaraki 305\u20108506 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Anh Thi","family":"Hoang","sequence":"additional","affiliation":[{"name":"Integrated Physiology Section, Department of Basic Medical Science National Institute for Minamata Disease  4058\u201018 Hama, Minamata Kumamoto 867\u20100008 Japan"},{"name":"Graduate School of Environmental and Symbiotic Science Prefectural University of Kumamoto  3\u20101\u2010100 Tsukide, Higashi\u2010ku Kumamoto 862\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Mogi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ehime University Graduate School of Medicine  Ehime 791\u20100295 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohisa","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Hoshi University School of Pharmacy and Pharmaceutical Sciences  Tokyo 142\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine National Institute for Minamata Disease  4058\u201018 Hama, Minamata Kumamoto 867\u20100008 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motohiro","family":"Takeya","sequence":"additional","affiliation":[{"name":"Department of Cell Pathology, Graduate School of Medical Sciences Kumamoto University  1\u20101\u20101 Honjo, Chuo\u2010ku Kumamoto 860\u20108556 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Komyo","family":"Eto","sequence":"additional","affiliation":[{"name":"Health and Nursing Facilities for the Aged Jushindai, Shinwakai  272 Ikurakitakata, Tamana Kumamoto 865\u20100041 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-7-714"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.12.041"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2786"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2013.09.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470942390.mo110165"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10072-012-1100-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1482-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2004.07.004"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230290166805"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.57.11.0017"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2011.05.019"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2014.05.017"},{"key":"e_1_2_7_14_1","article-title":"A simple composite phenotype scoring system for evaluating mouse models of cerebellar ataxia","volume":"21","author":"Guyenet S. 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