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Rosuvastatin reduces cholesterol and triglycerides; however, previous studies have shown that it may affect male reproduction. Ascorbic acid (AA), an antioxidant compound, plays a protective role in the male reproductive system. This study aimed to evaluate whether pre\u2010pubertal exposure to rosuvastatin may impair testicular structure and antioxidant status in male rats and if supplementation with AA may alleviate these damages. Male rats were randomly divided into six experimental groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) on postnatal day (PND) 23 and received the different treatments by gavage from PND 23 to 53. The experimental groups received vehicle (saline solution 0.9%), 3 or 10\u00a0mg\/kg\/day of rosuvastatin diluted in saline solution 0.9%, supplementation with 150\u00a0mg\/day of AA, 3\u00a0mg\/kg\/day of rosuvastatin in association with 150\u00a0mg\/day of AA or 10\u00a0mg\/kg\/day of rosuvastatin associated with 150\u00a0mg\/day of AA. Testicular parameters were assessed on PND 53 and 110. There were diminished androgen receptors staining in the Sertoli cells and increased germ cell death in rosuvastatin\u2010exposed groups, in both periods. Spermatids showed lower estrogen alpha\u2010receptors staining in the group exposed to 10\u00a0mg of statin at adulthood. There were androgen depletion and increased lipid peroxidation and catalase activity in statin\u2010exposed groups. Rosuvastatin exposure during pre\u2010puberty impaired testicular structure, steroid receptor distribution and increased oxidative stress; however, AA was able to ameliorate the impairment provoked by statin exposure.<\/jats:p>","DOI":"10.1002\/jat.3720","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T22:12:39Z","timestamp":1537567959000},"page":"305-321","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Ascorbic acid supplementation ameliorates testicular hormonal signaling, sperm production and oxidative stress in male rats exposed to rosuvastatin during pre\u2010puberty"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9437-6310","authenticated-orcid":false,"given":"Gabriel Adan Araujo","family":"Leite","sequence":"first","affiliation":[{"name":"Graduate Program in Cell and Structural Biology, Institute of Biology State University of Campinas \u2013 UNICAMP  Campinas Brazil"},{"name":"Department of Morphology, S\u00e3o Paulo State University (Unesp) Institute of Biosciences  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Willian Franco","family":"de Barros","sequence":"additional","affiliation":[{"name":"Department of Morphology, S\u00e3o Paulo State University (Unesp) Institute of Biosciences  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Airton da Cunha","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo \u2013 USP  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janete Aparecida","family":"Anselmo\u2010Franci","sequence":"additional","affiliation":[{"name":"Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeir\u00e3o Preto USP\u2014University of S\u00e3o Paulo, Ribeir\u00e3o Preto  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Fernando","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo \u2013 USP  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilma De Grava","family":"Kempinas","sequence":"additional","affiliation":[{"name":"Department of Morphology, S\u00e3o Paulo State University (Unesp) Institute of Biosciences  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-008-0344-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1990.tb02054.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.05016"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12461"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3989-03.2004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2007.08.010"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"issue":"2","key":"e_1_2_8_10_1","first-page":"77","article-title":"Statins and mitochondria","volume":"62","author":"Broniarek I.","year":"2016","journal-title":"Post\u0119py Biochemii"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2011.06.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000076816"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2011.09.003"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.20.3.0370"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308114100"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)86135-I"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.04.011"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh725"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2011.00688.x"},{"key":"e_1_2_8_21_1","first-page":"1","article-title":"Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats","volume":"100","author":"Fernandes G. 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