{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T17:28:51Z","timestamp":1762018131189,"version":"build-2065373602"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T00:00:00Z","timestamp":1538092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biofluid\u2010based biomarkers provide an efficient tool for hazard identification of chemicals. Here, we explored the potential of microRNAs (miRNAs) as biomarkers for hepatotoxicity of chemicals by linking in vitro to in vivo animal models. A search of the literature identified candidate circulating miRNA biomarkers of chemical\u2010induced hepatotoxicity. The expression of candidate miRNAs (miR\u2010122, miR\u2010151a, miR\u2010192, miR\u2010193a, miR\u2010194, miR\u201021, miR\u201029c), was determined by real\u2010time reverse transcription\u2010polymerase chain reaction in in vivo acute liver injury induced by acetaminophen, and then were further compared with those of in vitro cell assays. Candidate miRNAs, except miR\u201029c, were significantly or biologically upregulated by acetaminophen, at a dose that caused acute liver injury as confirmed by hepatocellular necrosis. Except miR\u2010122 and miR\u2010193a, other miRNAs elevated in in vivo models were confirmed by in vitro models using HepG2 cells, whereas they failed by in vitro models using human primary hepatocytes. These findings indicate that certain miRNAs may still have the potential of toxicological biomarkers in linking in vitro to in vivo hepatotoxicity.<\/jats:p>","DOI":"10.1002\/jat.3722","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T03:30:26Z","timestamp":1538105426000},"page":"333-342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of microRNA expressions for the identification of chemical hazards in in vivo and in vitro hepatic injury models"],"prefix":"10.1002","volume":"39","author":[{"given":"So\u2010Ryeon","family":"Hwang","sequence":"first","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nga Thi Thu","family":"Tham","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo\u2010ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hyun","family":"Bang","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee","family":"Yi","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010Il","family":"Park","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun\u2010Kyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwan\u2010Goo","family":"Kang","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Sang","family":"Kim","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gye\u2010Hyeong","family":"Woo","sequence":"additional","affiliation":[{"name":"Laboratory of Histopathology, Department of Clinical Laboratory Science Semyung University  Jecheon 27136 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7890-7919","authenticated-orcid":false,"given":"Hyun\u2010Ok","family":"Ku","sequence":"additional","affiliation":[{"name":"Toxicological Evaluation Laboratory Animal and Plant Quarantine Agency  177 Gimcheon 39660 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26294"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25873"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2014.03.006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.2013.827170"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2016.174"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfv260"},{"issue":"6","key":"e_1_2_7_9_1","first-page":"1428","article-title":"Circulating microRNA\u2010122 as a potential biomarker for liver injury","volume":"5","author":"Ding X.","year":"2012","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_7_10_1","unstructured":"EMEA. 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