{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T15:05:12Z","timestamp":1782140712957,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T00:00:00Z","timestamp":1546819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The extensive use of copper oxide nanoparticles (CuO\u2010NPs) in various industries and their wide range of applications have led to their accumulation in different ecological niches of the environment. This excess exposure raises the concern about its potential toxic effects on various organisms including humans. However, the hazardous potential of CuO\u2010NPs in the literature is elusive, and it is essential to study its toxicity in different biological models. Hence, we have conducted single acute dose (2000\u00a0mg\/kg) and multiple dose subacute (30, 300 and 1000\u00a0mg\/kg daily for 28\u00a0days) oral toxicity studies of CuO\u2010NPs in female albino Wistar rats following OECD guidelines 420 and 407 respectively. Blood analysis, tissue aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and acetylcholinesterase, superoxide dismutase, catalase, lipid malondialdehyde and reduced glutathione assays, and histopathology of the tissues were carried out. The higher dose treatments of the acute and subacute study caused significant alterations in biochemical and antioxidant parameters of the liver, kidney and brain tissues of the rat. In addition, histopathological evaluation of these three organs of treated rats showed significantly high abnormalities in their histoarchitecture when compared to control rats. We infer from the results that the toxicity observed in the liver, kidney and brain of treated rats could be due to the increased generation of reactive oxygen species by CuO\u2010NPs.<\/jats:p>","DOI":"10.1002\/jat.3760","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T21:49:24Z","timestamp":1546984164000},"page":"702-716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Acute and subacute oral toxicity of copper oxide nanoparticles in female albino Wistar rats"],"prefix":"10.1002","volume":"39","author":[{"given":"Lakshmi Sai Pratyusha","family":"Bugata","sequence":"first","affiliation":[{"name":"Toxicology Unit, Applied Biology Division CSIR\u2010Indian Institute of Chemical, Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabhakar","family":"Pitta Venkata","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Applied Biology Division CSIR\u2010Indian Institute of Chemical, Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananth Reddy","family":"Gundu","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Applied Biology Division CSIR\u2010Indian Institute of Chemical, Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahman","family":"Mohammed Fazlur","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Applied Biology Division CSIR\u2010Indian Institute of Chemical, Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utkarsh A.","family":"Reddy","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Applied Biology Division CSIR\u2010Indian Institute of Chemical, Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerald Mahesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"CSIR\u2010Centre for Cellular and Molecular Biology (CCMB)  Hyderabad, Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venugopala Reddy","family":"Mekala","sequence":"additional","affiliation":[{"name":"Andhra University College of Pharmaceutical Sciences  Visakhapatnam Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreedhar","family":"Bojja","sequence":"additional","affiliation":[{"name":"Analytical Division CSIR\u2010Indian Institute of Chemical Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6148-6038","authenticated-orcid":false,"given":"Mohammed","family":"Mahboob","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Applied Biology Division CSIR\u2010Indian Institute of Chemical, Technology  Hyderabad Telangana 500007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","unstructured":"Aebi H.(1984).[13] Catalase in vitro. (pp.121\u2013126).http:\/\/doi.org\/10.1016\/S0076\u20106879(84)05016\u20103","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.2003.tb12032.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-017-1170-4"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apcatb.2005.06.015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0048-3575(89)90003-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3392"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-3334-x"},{"key":"e_1_2_8_9_1","first-page":"63","volume-title":"Zeta Potential Measurement","author":"Clogston J. D.","year":"2011"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/1354750X.2017.1360943"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/1921216a0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3224"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2015.09.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2015.05.001"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000136485"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(84)90096-9"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480545.2012.720988"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-016-0169-x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11249-004-8109-6"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12274-014-0618-5"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS2001134"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2017.03.013"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2017.09.005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22526"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03714.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/NSA.S91785"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.21931"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2009.07.007"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2014.03.011"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114397"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-2-8"},{"key":"e_1_2_8_33_1","unstructured":"OECD. (2008).Test No. 407: Repeated Dose 28\u2010day Oral Toxicity Study in Rodents. (2008). OECD Publishing.http:\/\/doi.org\/10.1787\/9789264070684\u2010en"},{"key":"e_1_2_8_34_1","unstructured":"OECD. (2002).Test No. 420: Acute Oral Toxicity\u2014Fixed Dose Procedure. (2002). OECD Publishing.http:\/\/doi.org\/10.1787\/9789264070943\u2010en"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3303"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfj099"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1775"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22263"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2015.09.029"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18102115"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.09.019"},{"key":"e_1_2_8_42_1","first-page":"1505","article-title":"Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat","volume":"9","author":"Sarhan O. M. M.","year":"2014","journal-title":"International Journal of Nanomedicine"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2014.8752"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-009-9395-7"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2013.04.003"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacme.2015.09.005"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1648(01)00860-2"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr800545q"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfm279"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1130333"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/186079a0"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/18\/27\/275701"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.3760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.3760","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:27:49Z","timestamp":1760542069000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.3760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,7]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1002\/jat.3760"],"URL":"https:\/\/doi.org\/10.1002\/jat.3760","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,7]]},"assertion":[{"value":"2018-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}