{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T11:17:11Z","timestamp":1792840631767,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,3]],"date-time":"2019-11-03T00:00:00Z","timestamp":1572739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ethical issues in animal toxicity testing have led to the search for alternative methods to determine the skin sensitization potential of cosmetic products. The emergence of ethical testing issues has led to the development of many alternative methods that can reliably estimate skin sensitization potentials. However, a single alternative method may not be able to achieve high predictivity due to the complexity of the skin sensitization mechanism. Therefore, several prediction assays, including both in chemico and in vitro test methods, were investigated and integrated based on the skin sensitization adverse outcome pathway. In this study, we evaluated three different integrated approaches to predict a human skin sensitization hazard using data from in vitro assays (KeratinoSens\u2122 and human cell line activation test [h\u2010CLAT]), and a newly developed in chemico assay (spectrophotometric direct peptide reactivity assay [Spectro\u2010DPRA]). When the results of the in chemico and in vitro assays were combined, the predictivity of human data increased compared with that of a single assay. The highest predictivity was obtained for the approach in which sensitization potential was determined by Spectro\u2010DPRA followed by final determination using the result of KeratinoSens\u2122 and h\u2010CLAT assays (96.3% sensitivity, 87.1% specificity, 86.7% positive predictive value, 96.4% negative predictive value and 91.4% accuracy compared with human data). While further optimization is needed, we believe this integrated approach may provide useful predictive data when determining the human skin sensitization potential of chemicals.<\/jats:p>","DOI":"10.1002\/jat.3904","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T02:14:11Z","timestamp":1572833651000},"page":"300-312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Application of Spectro\u2010DPRA, KeratinoSens\u2122 and h\u2010CLAT to estimation of the skin sensitization potential of cosmetics ingredients"],"prefix":"10.1002","volume":"40","author":[{"given":"Sun\u2010A","family":"Cho","sequence":"first","affiliation":[{"name":"Safety &amp; Microbiology Research Lab, AmorePacific Corporation R&amp;D Unit  Yongin\u2010si Republic of Korea"},{"name":"Department of Laboratory Animal Medicine, Research Institute for Veterinary Science, BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minseok","family":"Choi","sequence":"additional","affiliation":[{"name":"Safety &amp; Microbiology Research Lab, AmorePacific Corporation R&amp;D Unit  Yongin\u2010si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sae\u2010Ra","family":"Park","sequence":"additional","affiliation":[{"name":"Safety &amp; Microbiology Research Lab, AmorePacific Corporation R&amp;D Unit  Yongin\u2010si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susun","family":"An","sequence":"additional","affiliation":[{"name":"Safety &amp; Microbiology Research Lab, AmorePacific Corporation R&amp;D Unit  Yongin\u2010si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4971-4640","authenticated-orcid":false,"given":"Jae\u2010Hak","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Medicine, Research Institute for Veterinary Science, BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2010.03764.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-011-0693-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2012.06.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2012.05.013"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2019.07.014"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2013.12.007"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1979.10"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2009.04.005"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2008.01477.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(80)90099-1"},{"key":"e_1_2_6_13_1","unstructured":"EU. (2006). Regulation (EC) No 1907\/2006 of the European Parliament and of the Council of18 December 2006 concerning the Registration Evaluation Authorization and Restriction of Chemicals (REACH) establishing a European Chemicals Agency amending Directive 1999\/45\/EC and repealing Council Regulation (EEC) No 793\/93 and Commission Regulation (EC) No1488\/94 as well as Council Directive 76\/769\/EEC and Commission Directives 91\/155\/EEC 93\/67\/EEC 93\/105\/EC and2000\/21\/EC. Official J. No L 396 1\u20101355."},{"key":"e_1_2_6_14_1","unstructured":"EU. (2008). Regulation (EC) No 1272\/2008 of the European Parliament and of the Council of 16 December 2008 on classification labelling and packaging of substances and mixtures amending 15 and repealing Directives 67\/548\/EEC and 1999\/45\/EC and amending Regulation (EC) No 1907\/2006. Off. J. No L 353 1\u20101355."},{"key":"e_1_2_6_15_1","unstructured":"EU. (2009). Regulation (EC) No 1223\/2009 of the European Parliament and of the Council of 30 November 2009 on cosmetic products. Off. J. No L 342 1\u201059."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.2310\/6620.2005.05040"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3558"},{"key":"e_1_2_6_18_1","unstructured":"ICCVAM. (2010). ICCVAM Test Method Evaluation Report on the Murine Local Lymph Node Assay: DA A Nonradioactive Alternative Test Method to Assess the Allergic Contact Dermatitis Potential of Chemicals and Products. NIH Publication Number 10\u20107551. National Toxicology Program."},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2869"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.14573\/altex.2011.3.211"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-015-1634-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00173-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq346"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/026119291204000310"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.61.5.643"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2016.01.009"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2018.11.001"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfp228"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfm259"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2868"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2012.07.001"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2012.11.006"},{"key":"e_1_2_6_33_1","unstructured":"OECD. (2012). The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins. Part 1: Scientific Evidence Series on Testing and Assessment No.168 JT03321047. Organisation for Economic Co\u2010operation and Development (OECD). Paris France."},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2017.06.002"},{"issue":"3","key":"e_1_2_6_35_1","first-page":"7","article-title":"Final report on the safety assessment of ammonium, potassium, and sodium persulfate","volume":"20","author":"Pang S.","year":"2001","journal-title":"International Journal of Toxicology"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2001.044006331.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3479"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2005.10.014"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2010.05.012"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2009.02.004"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3127"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2014.12.008"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.40.137"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3904","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.3904","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3904","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:33:44Z","timestamp":1760542424000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.3904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,3]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/jat.3904"],"URL":"https:\/\/doi.org\/10.1002\/jat.3904","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,3]]},"assertion":[{"value":"2019-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}