{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:21:19Z","timestamp":1787066479195,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Photoallergy test of cosmetics and several types of pharmaceutical substances is often necessary for obtaining approval from authorities. However, there are no official test guidelines for photoallergy evaluation. Therefore, we tried to establish a photoallergy test by utilizing an in chemico alternative sensitization method, amino acid derivative reactivity assay (ADRA). To determine the criteria for judging the photoallergy potential, photo\u2010ADRA with or without photoirradiation was performed using 60 photoallergenic chemicals, and cysteine and lysine derivatives were detected using high\u2010performance liquid chromatography either by absorbance or fluorescence measurement. The accuracy of prediction was 81.4% (48 of 59) and 80.0% (48 of 60) using the absorbance and fluorescence methods, respectively. However, as chemicals can breakdown into multiple chemicals during photoirradiation, the absorbance method often cannot perform accurate detection due to co\u2010elution, whereas the fluorescence method can do this due to lack of co\u2010elution. Moreover, all eight chemicals that were found to be negative or false\u2010positive for photoirritation in the 3T3 neutral red uptake phototoxicity test were confirmed as positive for photoallergy using this method. Furthermore, we prepared three types of pseudo\u2010mixtures where we added one photoallergen along with five nonphotoallergens and performed the photo\u2010ADRA by the ultraviolet and fluorescence methods. The result of the fluorescence method was almost the same as that obtained with the use of a single photoallergen and hence the outcome was not affected by the mixture. Thus, this study not only showed a method of evaluating the photoallergy potential of a single chemical but also a mixture, making it useful as an in chemico photoallergy alternative test.<\/jats:p>","DOI":"10.1002\/jat.3935","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T21:39:53Z","timestamp":1583271593000},"page":"655-678","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Development of photo\u2010amino acid derivative reactivity assay: a novel in chemico alternative method for predicting photoallergy"],"prefix":"10.1002","volume":"40","author":[{"given":"Yusuke","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Safety Evaluation Center, Fujifilm Corporation  Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4345-1154","authenticated-orcid":false,"given":"Masaharu","family":"Fujita","sequence":"additional","affiliation":[{"name":"Safety Evaluation Center, Fujifilm Corporation  Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Wanibuchi","sequence":"additional","affiliation":[{"name":"Safety Evaluation Center, Fujifilm Corporation  Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Sato","sequence":"additional","affiliation":[{"name":"Safety Evaluation Center, Fujifilm Corporation  Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Katsuoka","sequence":"additional","affiliation":[{"name":"Safety Evaluation Center, Fujifilm Corporation  Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7115-8452","authenticated-orcid":false,"given":"Toshihiko","family":"Kasahara","sequence":"additional","affiliation":[{"name":"Safety Evaluation Center, Fujifilm Corporation  Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.110.034546"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.04.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/11592780-000000000-00000"},{"key":"e_1_2_8_5_1","unstructured":"European Commission(2011).Opinion on Methyl\u2010N\u2010methylanthranilate. Scientific Committee on Consumer Safetyhttps:\/\/ec.europa.eu\/health\/scientific_committees\/comsumer_safety\/docs\/sccs_o_075.pdf"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569527.2012.656293"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2014.06.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2019.02.004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3707"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2019.04.014"},{"key":"e_1_2_8_11_1","unstructured":"IARC Monographs. (2016).Sulfasalazine.https:\/\/monographs.iarc.fr\/wp-content\/uploads\/2018\/06\/mono108-08.pdf."},{"key":"e_1_2_8_12_1","unstructured":"ICH S10 Guideline. (2014)Photosafety evaluation of pharmaceuticals.http:\/\/www.ich.org\/fileadmin\/Public_Web_Site\/ICH_Products\/Guidelines\/Safety\/S10\/S10_Step_4.pdf."},{"key":"e_1_2_8_13_1","unstructured":"ICHM3 (R2) Guideline. (2009)Non\u2010Clinical Safety Studies for the Conduct of Human Clinical Trials and Marketing Authorization for Pharmaceuticals.www.ich.org\/fileadmin\/Public_Web_Site\/ICH__Products\/Guidelines\/Multidisciplinary\/M3_R2\/Step4\/M3_R2__Guideline.pdf."},{"issue":"7","key":"e_1_2_8_14_1","first-page":"676","article-title":"Oxidation of a metabolite of indomethacin (desmethyldeschlorobenzoylindomethacin) to reactive intermediates by activated neutrophils, hypochlorous acid, and the myeloperoxidase system","volume":"26","author":"Ju C.","year":"1998","journal-title":"Drug Metabolism and Disposition"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gem053"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(98)00189-4"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200225050-00004"},{"key":"e_1_2_8_18_1","unstructured":"OECD. (2004).In vitro 3T3 NRU photo toxicity testhttps:\/\/www.oecd-ilibrary.org\/docserver\/9789264071162-en.pdf?expires=1555907736&id=id&accname=guest&checksum=87E7827789EE1166CF05824AB4C6302B"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-1873.2004.0378b.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/169432"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2016.03.003"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2013.10.003"},{"issue":"3","key":"e_1_2_8_23_1","first-page":"373","article-title":"Comparison of in chemico skin sensitization methods and development of an in chemico skin photosensitization assay","volume":"36","author":"Patel N. H.","year":"2019","journal-title":"ALTEX"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13142"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12953"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2013.05.008"},{"issue":"2","key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/S0022-3565(25)32069-0","article-title":"Isoniazid hepatoxicity: the relationship between covalent binding and metabolism in vivo","volume":"213","author":"Timbrell J. A.","year":"1980","journal-title":"The Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.43.247"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3234"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2014.12.008"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2019.106624"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.vascn.2019.01.001","article-title":"Expanding the applicability of the Amino acid Derivative Reactivity Assay (ADRA): Determining a weight concentration for preparation of test chemical solutions that yields a predictive capacity identical to the conventional method using molar concentration and demonstrating the capacity to detect sensitizers in liquid mixtures","volume":"21","author":"Yamamoto Y.","year":"2019","journal-title":"Journal of Pharmacological and Toxicological Methods"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.44.585"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3139"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.3935","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3935","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.3935","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:47:27Z","timestamp":1760557647000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.3935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,3]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/jat.3935"],"URL":"https:\/\/doi.org\/10.1002\/jat.3935","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,3]]},"assertion":[{"value":"2020-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}