{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:11:03Z","timestamp":1790507463031,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bisphenol A (BPA), a very important raw material in the plastics industry, is an endocrine\u2010disrupting chemical in teleost fish. Although BPA induces testis\u2010ova and sex reversal in teleost fish species, the molecular mechanism remains unclear. We evaluated the effects of BPA (measured concentrations: 45, 92, 326, 1030 and 3406 \u03bcg\/L) on Japanese medaka (<jats:styled-content style=\"fixed-case\"><jats:italic>Oryzias latipes<\/jats:italic><\/jats:styled-content>) using OECD TG234 (2011, <jats:italic>Fish Sexual Development Test<\/jats:italic>, OECD Guidelines for the Testing of Chemicals, Section 2). BPA at 1030 and 3406 \u03bcg\/L induced testis\u2010ova and sex reversal with female\u2010type secondary sexual characteristics in XY males at 30 and 60 days posthatching (dph). Then we examined the BPA effect on the expression of sex differentiation genes related to the testis\u2010ova and sex reversal in XY medaka. BPA exposure (1030 and 3406 \u03bcg\/L) suppressed <jats:italic>gsdf<\/jats:italic> mRNA expression and increased <jats:italic>cyp19a1a<\/jats:italic> mRNA expression in XY individuals at stage 38 and 30 dph, although <jats:italic>foxl2<\/jats:italic> mRNA expression showed no change. Interestingly, the concentration of BPA that suppressed <jats:italic>gsdf<\/jats:italic> mRNA expression at the larval stage was consistent with that needed to induce testis\u2010ova and sex reversal. These results suggest that the <jats:italic>gsdf<\/jats:italic> gene at the embryonic stage can be used as a useful biomarker for predicting the impact of estrogenic endocrine\u2010disrupting chemicals on sexual differentiation in Japanese medaka.<\/jats:p>","DOI":"10.1002\/jat.3945","type":"journal-article","created":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T22:32:33Z","timestamp":1580855553000},"page":"804-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Bisphenol A induces a shift in sex differentiation gene expression with testis\u2010ova or sex reversal in Japanese medaka (<scp><i>Oryzias latipes<\/i><\/scp>)"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4558-8624","authenticated-orcid":false,"given":"Yoshifumi","family":"Horie","sequence":"first","affiliation":[{"name":"Faculty of Bioresource Sciences Akita Prefectural University  Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuhiko","family":"Kanazawa","sequence":"additional","affiliation":[{"name":"Faculty of Systems Science and Technology Akita Prefectural University  Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiho","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Faculty of Bioresource Sciences Akita Prefectural University  Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihisa","family":"Tatarazako","sequence":"additional","affiliation":[{"name":"Department of Science and Technology for Biological Resources and Environment Graduate School of Agriculture  Matsuyama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisen","family":"Iguchi","sequence":"additional","affiliation":[{"name":"Nanobioscience Yokohama City University  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2005.02.100"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2004.03.018"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2016.11.089"},{"key":"e_1_2_8_5_1","first-page":"263","article-title":"Factors initiating mating behavior and oviposition in the fish, Oryzias latipes","volume":"9","author":"Egami N.","year":"1961","journal-title":"Journal of the Faculty Science, University of Tokyo. Sect. Iv"},{"key":"e_1_2_8_6_1","first-page":"33","article-title":"Directive 2010\/63\/EU of the European Parliament and Council of 22 September on the protection of animals used for scientific purposes","volume":"276","author":"EU.","year":"2010","journal-title":"The Official Journal of the European Union"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.3923"},{"key":"e_1_2_8_8_1","article-title":"Rcmdr: R Commander","volume":"2","author":"Fox J.","year":"2018","journal-title":"R Package Version"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-008-0474-4"},{"key":"e_1_2_8_10_1","first-page":"242","article-title":"Contamination of estrogenic compounds in the surface water of Guangzhou reach of the Pearl River","volume":"27","author":"Gong J.","year":"2008","journal-title":"Environmental Chemistry"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/36.11.565"},{"key":"e_1_2_8_12_1","unstructured":"Grand View Research. (2014).https:\/\/www.grandviewresearch.com\/industry-analysis\/bisphenol-a-bpa-market."},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401730306"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2016.07.022"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2017.08.016"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3365"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.03.135"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2011.04.010"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.064246"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.09.112"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19990201)283:2<210::AID-JEZ12>3.0.CO;2-F"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2004.03.012"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5620211119"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-005-0896-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2007.01.009"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jes.2015.08.006"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2014.06.035"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.22.1163"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2017.08.011"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20158"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-1136(00)00068-4"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2015.03.025"},{"key":"e_1_2_8_33_1","first-page":"30","article-title":"Distribution characteristics of bisphenol A in Shihwa Lake and nearby creeks","volume":"2","author":"Li Z. Y.","year":"2004","journal-title":"Transactions of Oceanology and Limnology"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.09.035"},{"key":"e_1_2_8_35_1","first-page":"1","article-title":"Investigation of several endocrine disrupting chemicals in Huangpu River and water treatment units of a waterworks","volume":"22","author":"Ma X. Y.","year":"2006","journal-title":"China Water Wastewater."},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature751"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.1976"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.137497"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.03.137"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.07.013"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1787\/9789264122369-en"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1787\/9789264203709-en"},{"key":"e_1_2_8_43_1","first-page":"209","article-title":"On the processed on the fin\u2010rays of the male of Oryzias latipes and other sex characters off this fish","volume":"2","author":"Oka T. B.","year":"1931","journal-title":"Journal of the Faculty of Science, Imperial University of Tokyo Section, IV Zoology"},{"key":"e_1_2_8_44_1","first-page":"383","article-title":"Experimental investigation of the manifestation of secondary sexual characters in fish, using the medaka, Oryzias latipes (Temminck & Schlegel) as material","volume":"6","author":"Okada Y. K.","year":"1944","journal-title":"Journal of the Faculty of Science, Imperial University of Tokyo Section, IV Zoology"},{"key":"e_1_2_8_45_1","first-page":"197","article-title":"Mating ethogram in Oryzias latipes","volume":"13","author":"Ono Y.","year":"1957","journal-title":"Journal of the Faculty of Science, Hokkaido University. Series. VI Zoology"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.01947"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfl051"},{"issue":"10","key":"e_1_2_8_48_1","first-page":"2723","article-title":"Investigation of endocrine disrupting chemicals in a drinking water work located in Songhua River basin","volume":"29","author":"Shao X. L.","year":"2008","journal-title":"Huan Jing Ke Xue=Huanjing Kexue\/ [Bian Ji, Zhongguo Ke Xue Yuan Huan Jing Ke Xue Wei Yuan Hui \"Huan Jing Ke Xue\" Bian Ji Wei Yuan Hui.]"},{"key":"e_1_2_8_49_1","first-page":"1","article-title":"NTP\u2010CERHR monograph on the potential human reproductive and developmental effects of bisphenol A","volume":"22","author":"Shelby M. D.","year":"2008","journal-title":"National Toxicology Program, Center For The Evaluation of Risks To Human Reproduction, MON"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2010.06.005"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0045-6535(99)00235-0"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-014-9980-1"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0269-7491(02)00483-9"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2011.05.010"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-014-1298-2"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.a.20027"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1971.00119.x"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.05.133"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0248"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.3895"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2004.05.017"},{"key":"e_1_2_8_62_1","first-page":"62","article-title":"Determination of bisphenol A, nonylphenol, octylphenol in water by liquid chromatography\u2010tandem mass spectrometry","volume":"27","author":"Zhang K. W.","year":"2008","journal-title":"Chinese Journal Analytical Chemistry"},{"issue":"8","key":"e_1_2_8_63_1","first-page":"2634","article-title":"Mutation of foxl2 or cyp19a1a results in female to male sex reversal in XX Nile tilapia","volume":"158","author":"Zhang X.","year":"2017","journal-title":"Endocrinology"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2017.07.032"},{"key":"e_1_2_8_65_1","first-page":"3590","article-title":"Evaluation of estrogenicity of sewage samples from Beijing","volume":"30","author":"Zhou H. D.","year":"2009","journal-title":"Chinese Environmental Science"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.3945","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3945","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.3945","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T09:25:52Z","timestamp":1760520352000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.3945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,4]]},"references-count":64,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/jat.3945"],"URL":"https:\/\/doi.org\/10.1002\/jat.3945","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,4]]},"assertion":[{"value":"2019-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}