{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:01Z","timestamp":1786977901308,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T00:00:00Z","timestamp":1583884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the 13th Five-Year National Key R&D Program of China","award":["2018YFC0807202-3"],"award-info":[{"award-number":["2018YFC0807202-3"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Early diagnosis of cantharidin\u2010induced myocardial injury is the key to reduce the fatality rate in clinical practice. The purpose of the present study was to explore biomarkers that can be used for the prediction and diagnosis of cantharidin\u2010induced myocardial injury. Of 65 male Sprague\u2010Dawley rats weighing 200\u2010230 g, 25 rats were divided into five groups according to the administration dose of cantharidin (0, 1.34, 2.67, 4 and 5.34 mg\/kg;\n                    <jats:italic>n<\/jats:italic>\n                    = 5 per group) and the other 40 rats were treated with 2.67 mg\/kg cantharidin and divided into nine groups according to the administration time (0, 1, 2, 4, 6, 8, 12, 24, 48 and 72 hours;\n                    <jats:italic>n<\/jats:italic>\n                    = 4 per group). Pathological changes of hypoxia, necrosis and inflammation were confirmed in heart samples that were exposed to cantharidin by hematoxylin\u2010eosin staining and overall scores of pathological changes among heart samples in cantharidin exposure groups showed an increasing trend compared with in the control group. Coexpression of vascular endothelial growth factor (VEGF), hypoxia inducible factor\u20101\u03b1 (HIF\u20101\u03b1) and caspase9 was shown in the myocardium by immunofluorescence staining. Western blotting results showed that expression of VEGF, HIF\u20101\u03b1 and caspase9 in cantharidin\u2010treated rat hearts showed an increasing trend compared with in the control group. Results of enzyme\u2010linked immunosorbent assay suggested that plasma levels of troponin T (TN\u2010T), VEGF and HIF\u20101\u03b1 were elevated at different intervals after cantharidin administration, and VEGF and HIF\u20101\u03b1 had a significant linear relationship with TN\u2010T that was verified by multiple linear regression analysis. Preliminary results serve to illustrate that TN\u2010T, VEGF and HIF\u20101\u03b1 might be valuable molecular markers in cantharidin\u2010induced myocardial injury and that diagnostic accuracy needs to be studied further.\n                  <\/jats:p>","DOI":"10.1002\/jat.3974","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T00:50:15Z","timestamp":1583974215000},"page":"1153-1161","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Molecular biomarkers of cantharidin\u2010induced cardiotoxicity in Sprague\u2010Dawley rats: Troponin T, vascular endothelial growth factor and hypoxia inducible factor\u20101\u03b1"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8371-8709","authenticated-orcid":false,"given":"Zhang","family":"Youyou","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4264-7166","authenticated-orcid":false,"given":"Yu","family":"Yalei","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Jie","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan","family":"Chuhuai","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8437-5998","authenticated-orcid":false,"given":"Liu","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2446-1544","authenticated-orcid":false,"given":"Ren","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-017-9556-7"},{"issue":"2","key":"e_1_2_9_3_1","first-page":"111","article-title":"Cantharidin in dermatology","volume":"14","author":"Al\u2010Dawsari N. 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