{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:42:43Z","timestamp":1760575363542,"version":"build-2065373602"},"reference-count":12,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Globally Harmonized System of Classification and Labelling of Chemicals (GHS) is a hazard classification and communication system for providing information on the safe handling of chemicals worldwide. In this study, we evaluated the applicability of the newly proposed GHS subcategorization criterion for murine local lymph node assay:2\u2010bromodeoxyuridine enzyme\u2010linked immunosorbent assay (LLNA:BrdU\u2010ELISA), Category 1A:EC1.6 \u22646%, Category 1B:EC1.6 &gt;6%, to data derived from LLNA:BrdU\u2010ELISA performed in the CBA\/J strain mouse. Fifteen chemicals categorized in GHS hazard Category 1 sensitizers listed in the LLNA performance standard were tested by LLNA:BrdU\u2010ELISA in the CBA\/J strain mouse and were classified according to the new criterion. The results revealed that all of the GHS 1A or 1B category chemicals classified according to the EC3 values derived from radioisotopic LLNA (LLNA\u2010RI) could be correctly assigned into the respective 1A and 1B categories using the newly proposed GHS subclassification criterion. In addition, analysis of the correlation between the reported EC3 values and EC1.6 values derived from the LLNA:BrdU\u2010ELISA performed in the CBA\/J strain mouse confirmed the existence of a strong correlation (<jats:italic>r<\/jats:italic> = 0.9076, <jats:italic>P<\/jats:italic> &lt; .0001). These findings suggest that the newly proposed GHS subcategorization criterion for LLNA:BrdU\u2010ELISA is potentially applicable for practical use in GHS subcategorization.<\/jats:p>","DOI":"10.1002\/jat.3996","type":"journal-article","created":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T23:48:08Z","timestamp":1588722488000},"page":"1435-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Applicability of the proposed GHS subcategorization criterion for LLNA:BrdU\u2010ELISA (OECD TG442B) to the CBA\/J strain mouse"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2783-0170","authenticated-orcid":false,"given":"Toshio","family":"Kobayashi","sequence":"first","affiliation":[{"name":"CERI Hita Chemicals Evaluation and Research Institute  Ishii\u2010machi, Hita\u2010shi Oita Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University  Yamaguchi\u2010shi Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-9851","authenticated-orcid":false,"given":"Yosuke","family":"Maeda","sequence":"additional","affiliation":[{"name":"The United Graduate School of Veterinary Science Yamaguchi University  Yamaguchi\u2010shi Yamaguchi Japan"},{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Shimotakano, Sugito\u2010machi, Kitakatsushika\u2010gun Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruka","family":"Kondo","sequence":"additional","affiliation":[{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Shimotakano, Sugito\u2010machi, Kitakatsushika\u2010gun Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Takeyoshi","sequence":"additional","affiliation":[{"name":"Chemicals Assessment and Research Center Chemicals Evaluation and Research Institute  Shimotakano, Sugito\u2010machi, Kitakatsushika\u2010gun Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,5]]},"reference":[{"volume-title":"Recommended performance standards: Murine local lymph node assay","year":"2009","author":"ICCVAM","key":"e_1_2_7_2_1"},{"key":"e_1_2_7_3_1","unstructured":"ICCVAM. (2010).ICCVAM test method evaluation report on the murine local lymph node assay: BrdU\u2010ELISA. A Nonradioactive Alternative Test Method to Assess the Allergic Contact Dermatitis Potential of Chemicals and Products. Research Triangle Park NC: NIEHS.https:\/\/ntp.niehs.nih.gov\/iccvam\/docs\/immunotox_docs\/llna-elisa\/tmer.pdf(accessed on March 31 2020)."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq346"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2019.104409"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3250"},{"key":"e_1_2_7_7_1","unstructured":"OECD. (2010).Test No. 429: Skin sensitisation: Local lymph node assay OECD Guidelines for the Testing of Chemicals Section 4. Paris: OECD Publishing. 10.1787\/9789264071100\u2010en."},{"key":"e_1_2_7_8_1","unstructured":"OECD. (2018).Test No. 442B: Skin sensitization: Local lymph node assay: BrdU\u2010ELISA or \u2010FCM (Appendix IA) OECD Guidelines for the Testing of Chemicals Section 4. Paris: OECD Publishing. 10.1787\/9789264090996\u2010en."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/15569520701212258"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1045"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(03)00255-5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(00)00315-5"},{"key":"e_1_2_7_13_1","first-page":"161","volume-title":"Globally Harmonized System of Classification and Labelling of Chemicals (GHS) 8th revised edition. Respiratory or skin sensitization","author":"United Nations","year":"2019"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.3996","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.3996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:23:28Z","timestamp":1760531008000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.3996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,5]]},"references-count":12,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1002\/jat.3996"],"URL":"https:\/\/doi.org\/10.1002\/jat.3996","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"type":"print","value":"0260-437X"},{"type":"electronic","value":"1099-1263"}],"subject":[],"published":{"date-parts":[[2020,5,5]]},"assertion":[{"value":"2020-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}