{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T18:48:28Z","timestamp":1774378108085,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,5,30]],"date-time":"2020-05-30T00:00:00Z","timestamp":1590796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nationally Key R&D Program of China","award":["2017YFC1200900"],"award-info":[{"award-number":["2017YFC1200900"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current study explores the detoxification effect of Retro\u20102 on ricin toxin (RT) cytotoxicity, as well as the mechanisms underlying such effects, to provide a basis for follow\u2010up clinical applications of Retro\u20102. The mouse\u2010derived mononuclear\/macrophage cell line, RAW264.7, was used to evaluate the detoxification effect of Retro\u20102 on RT by detecting cell viability, capacity for protein synthesis and the expression of cytokines, as well as endoplasmic reticulum stress (ERS)\u2010related mRNA. The results indicated that many cells died when challenged with concentrations of RT \u226550ng\/mL. The protein synthesis capacity of cells decreased when challenged with 200ng\/mL RT for 2hours. Furthermore, the synthesis and release of many cytokines decreased, while the expression of cytokines or ERS\u2010related mRNA increased when challenged with 200ng\/mL of RT for 12 or more hours. However, cell viability, capacity for protein synthesis and release levels of many cytokines were higher, while the expression levels of cytokine, or ERS\u2010related mRNA, were lower in cells pretreated with 20\u03bcm Retro\u20102 and challenged with RT, compared with those that had not been pretreated with Retro\u20102. In conclusion, Retro\u20102 retained the capacity for protein synthesis inhibited by RT, alleviated ERS induced by RT and increased the viability of cells challenged with RT. Retro\u20102 shows the potential for clinical applications.<\/jats:p>","DOI":"10.1002\/jat.3997","type":"journal-article","created":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T02:29:15Z","timestamp":1590892155000},"page":"1440-1450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pretreatment with Retro\u20102 protects cells from death caused by ricin toxin by retaining the capacity of protein synthesis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7102-3360","authenticated-orcid":false,"given":"Zhouguang","family":"Jiao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuehua","family":"Ke","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Chinese 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