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As a regulator of gene expression, microRNAs (miRNAs) were involved in some lung diseases. This study aimed to analyze the levels of some inflammatory cytokine and the differential expressions of miRNAs in human bronchial epithelial cells (16HBE) induced by <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.baidu.com\/link?url=GA9BdgG5lNTOupSxvr9We4VLIQPghG4zaH9FOUW5MficmPG1v_PSCaX_joyNnFo9D8nUjEFS5gFU5LIzC92mRjIOvLAX3o157mpTQPp1C_b4lT9pSSypmIrXipwl80zR%26wd=%26eqid=fe954ac100077f7f000000065b8a517a\">beryllium\u00a0sulfate<\/jats:ext-link> (BeSO<jats:sub>4<\/jats:sub>) and to further explore the biological functions of differentially expressed miRNAs. The profile of miRNAs in 16HBE cells was detected using the high\u2010throughput sequencing between the control groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a03) and the 150\u00a0\u03bcmol\/L of BeSO<jats:sub>4<\/jats:sub>\u2010treated groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a03). Bioinformatics analysis of differentially expressed miRNAs was performed, including the prediction of target genes, Gene Ontology (GO) analysis, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was applied to verify some damage\u2010related miRNAs. We found that BeSO<jats:sub>4<\/jats:sub> can increase the levels of some inflammatory cytokine such as interleukin\u201010 (IL\u201010), tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), interferon\u2010\u03b3 (IFN\u2010\u03b3), inducible nitric oxide synthase (iNOS), and cyclooxygenase\u20102 (COX\u20102). And BeSO<jats:sub>4<\/jats:sub> altered miRNAs expression of 16HBE cells and a total of 179 differentially expressed miRNAs were identified, including 88 upregulated miRNAs and 91 downregulated miRNAs. The target genes predicted by 28 dysregulated miRNAs were mainly involved in the transcription regulation, signal transduction, MAPK, and VEGF signaling pathway. The qRT\u2010PCR verification results were consistent with the sequencing results. miRNA expression profiling in 16HBE cells exposed to BeSO<jats:sub>4<\/jats:sub> provides new insights into the toxicity mechanism of beryllium exposure.<\/jats:p>","DOI":"10.1002\/jat.4116","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T09:53:50Z","timestamp":1605520430000},"page":"1275-1285","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The expression profile and bioinformatics analysis of microRNAs in human bronchial epithelial cells treated by beryllium sulfate"],"prefix":"10.1002","volume":"41","author":[{"given":"Shan","family":"Yi","sequence":"first","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010ping","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun\u2010ya","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010yan","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan\u2010di","family":"Lei","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6104-2197","authenticated-orcid":false,"given":"Zhao\u2010hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/get032"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-018-3541-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2018.03.006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17060842"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.06.023"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5278.55122"},{"issue":"2","key":"e_1_2_7_11_1","first-page":"879","article-title":"miR\u20106852 serves as a prognostic biomarker in colorectal cancer and inhibits tumor growth and metastasis by targeting TCF7","volume":"16","author":"Cui B. 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