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However, pesticides\u2010mediated toxicity in the endothelial cells (ECs) is still elusive. Hence, in this study, we have used human umbilical vein endothelial cells (HUVECs) as a model to quantify the cytotoxicity and genotoxicity of four pesticides (methomyl, carbaryl, metalaxyl, and pendimethalin). In the MTT assay, HUVECs exposed to methomyl, carbaryl, metalaxyl, and pendimethalin demonstrated significant proliferation inhibition only at higher concentrations (500 and 1000 \u03bcM). Likewise, neutral red uptake (NRU) assay also showed proliferation inhibition of HUVECs at 500 and 1000 \u03bcM by the four pesticides, confirming lysosomal fragility. HUVECs exposed to the four pesticides significantly increased the level of intracellular reactive oxygen species (ROS). Comet assay and flow cytometric data exhibited DNA damage and apoptotic cell death in HUVECs after 24 h of exposure with methomyl, metalaxyl, carbaryl, and pendimethalin. This is a first study on HUVECs signifying the cytotoxic\u2013genotoxic and apoptotic potential of carbamate insecticides (methomyl and carbaryl), fungicide (metalaxyl), and herbicide (pendimethalin). Overall, these pesticides may affect ECs functions and angiogenesis; nonetheless, mechanistic studies are warranted from the perspective of vascular biology using in vivo test models.<\/jats:p>","DOI":"10.1002\/jat.4139","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:43:03Z","timestamp":1610671383000},"page":"832-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-3777","authenticated-orcid":false,"given":"Quaiser","family":"Saquib","sequence":"first","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2010-3088","authenticated-orcid":false,"given":"Maqsood A.","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"DNA Research Chair, Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabiha M.","family":"Ansari","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hend A.","family":"Alwathnani","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javed","family":"Musarrat","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Sciences, Department of Agricultural Microbiology Aligarh Muslim University  Aligarh Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz A.","family":"Al\u2010Khedhairy","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2020.03.030"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.5923\/j.ajb.20120205.03"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwh290"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9789241"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24185"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2017.04.013"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-017-1622-0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.126644"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2018.03.009"},{"issue":"5","key":"e_1_2_8_11_1","first-page":"322","article-title":"Sub\u2010acute toxicity studies on pendimethalin in rats","volume":"29","author":"Ayub S. 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