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Circular RNAs (circRNAs) play a crucial role in disease, especially cardiovascular disease. We aimed to explore the circRNA expression profiles and potential mechanisms during CsA\u2010induced cardiotoxicity. Sixty male adult Wistar rats were randomly divided into two groups. The CsA group was injected with CsA (15\u2009mg\/kg\/day body weight) intraperitoneally (ip) for 2\u00a0weeks, whereas the control group was injected ip with the same volume of olive oil. We assessed CsA\u2010induced cardiotoxicity by light microscopy, transferase\u2010mediated dUTP nick\u2010end labeling (TUNEL) staining, and electron microscopy. Microarray analysis was used to detect the expression profiles of circRNAs deregulated in the heart during CsA\u2010induced cardiotoxicity. We confirmed the changes in circRNAs by quantitative PCR. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of the microarray data were performed. A conventional dose of CsA induced cardiotoxicity in rats. We identified 67 upregulated and 37 downregulated circRNAs compared with those in the control group. Six of 12 circRNAs were successfully verified by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR). GO analyses of the differentially expressed circRNAs indicated that these molecules might play important roles in CsA\u2010induced cardiotoxicity. KEGG pathway analyses showed that the differentially expressed circRNAs in CsA\u2010induced cardiotoxicity may be related to autophagy or the Hippo signaling pathway. We identified differential circRNA expression patterns and provided more insight into the mechanism of CsA\u2010induced cardiotoxicity. CircRNAs may serve as potential biomarkers or therapeutic targets of CsA\u2010mediated cardiotoxicity in the future.<\/jats:p>","DOI":"10.1002\/jat.4203","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T00:50:03Z","timestamp":1621990203000},"page":"216-229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Potential role of circular RNA in cyclosporin A\u2010induced cardiotoxicity in rats"],"prefix":"10.1002","volume":"42","author":[{"given":"Yanru","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Cardiology The Fourth Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology The Fourth Affiliated Hospital of Harbin Medical University  Harbin 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