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Hydrogen sulfide has been found to protect cells. The present study aimed to determine the protective mechanisms involved in hydrogen sulfide treatment of 16HBE cells following beryllium sulfate\u2010induced injury. 16HBE cells were treated with beryllium sulfate doses ranging between 0 and 300 \u03bcM BeSO<jats:sub>4<\/jats:sub>. Additionally, 16HBE cells were subjected to pretreatment with either a 300 \u03bcM dose of sodium hydrosulfide (a hydrogen sulfide donor) or 10 mM DL\u2010propargylglycine (a cystathionine\u2010<jats:italic>\u03b3<\/jats:italic>\u2010lyase inhibitor) for 6\u2009hr before then being treated with 150 \u03bcM beryllium sulfate for 48\u2009hr. This study illustrates that beryllium sulfate induces a reduction in cell viability, increases lactate dehydrogenase (LDH) release, and increases cellular apoptosis and autophagy in 16HBE cells. Interestingly, pretreating 16HBE cells with sodium hydrosulfide significantly reduced the beryllium sulfate\u2010induced apoptosis and autophagy. Moreover, it increased the mitochondrial membrane potential and alleviated the G2\/M\u2010phase cell cycle arrest. However, pretreatment with 10 mM DL\u2010propargylglycine promoted the opposite effects. PI3K\/Akt\/mTOR and Nrf2\/ARE signaling pathways are also activated following pretreatment with sodium hydrosulfide. These results indicate the protection provided by hydrogen sulfide in 16HBE cells against beryllium sulfate\u2010induced injury is associated with the inhibition of apoptosis and autophagy through the activation of the PI3K\/Akt\/mTOR and Nrf2\/ARE signaling pathways. Therefore, hydrogen sulfide has the potential to be a promising candidate in the treatment against beryllium disease.<\/jats:p>","DOI":"10.1002\/jat.4205","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T00:10:17Z","timestamp":1623024617000},"page":"230-243","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Hydrogen sulfide alleviates apoptosis and autophagy induced by beryllium sulfate in 16HBE cells"],"prefix":"10.1002","volume":"42","author":[{"given":"Yan\u2010ping","family":"Liu","sequence":"first","affiliation":[{"name":"School of public health University of South China  Hengyang China"},{"name":"Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010yan","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of 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