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Herein, following 6\u2009months of exposure in mice to 300, 600, 1200 or 2400\u2009\u03bcg\/L iodine, the biochemical signature of alterations to the liver\/pancreas\/kidney was profiled using attenuated total reflection Fourier\u2010transform infrared (ATR\u2010FTIR) spectroscopy coupled with principal component analysis\u2010linear discriminant analysis (PCA\u2010LDA). Our research showed that serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (Scr), insulin, blood glucose levels and homeostasis model assessment for insulin resistance (HOMA\u2010IR) index in the 1200 and 2400\u2009\u03bcg\/L iodine\u2010treated groups were significantly increased compared with those in the control group. Moreover, histological analysis showed that the liver\/kidney\/pancreas tissues of mice exposed to EI treatment displayed substantial morphological abnormalities, such as a loss of hepatic architecture, glomerular cell vacuolation and pancreatic neutrophilic infiltration. Notably, EI treatment caused distinct biochemical signature segregation between EI\u2010exposed versus the control liver\/pancreas\/kidney. The main biochemical alterations between EI\u2010exposed and control groups were observed for protein phosphorylation, protein secondary structures and lipids. The ratios of amide I\u2010to\u2010amide II (1674\u2009cm<jats:sup>\u22121<\/jats:sup>\/1570\u2009cm<jats:sup>\u22121<\/jats:sup>), \u03b1\u2010helix\u2010to\u2010\u03b2\u2010sheet (1657\u2009cm<jats:sup>\u22121<\/jats:sup>\/1635\u2009cm<jats:sup>\u22121<\/jats:sup>), glycogen\u2010to\u2010phosphate (1030\u2009cm<jats:sup>\u22121<\/jats:sup>\/1086\u2009cm<jats:sup>\u22121<\/jats:sup>) and the peptide aggregation (1 630\u2009cm<jats:sup>\u22121<\/jats:sup>\/1650\u2009cm<jats:sup>\u22121<\/jats:sup>) level of EI\u2010treated groups significantly differed from the control group. Our study demonstrated that EI induced hepatic, renal and pancreatic injury by disturbing the structure, metabolism and function of the cell membrane. This finding provides the new method and implication for human health assessment regarding long\u2010term EI intake.<\/jats:p>","DOI":"10.1002\/jat.4242","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T06:09:20Z","timestamp":1632895760000},"page":"600-616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Iodine excess induces hepatic, renal and pancreatic injury in female mice as determined by attenuated total reflection Fourier\u2010transform infrared spectrometry"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1493-5838","authenticated-orcid":false,"given":"Yang","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Xiangyang City, Department of Obstetrics and Gynaecology, Xiangyang No.1 People's Hospital Hubei University of Medicine  Xiangyang 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