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Gender\u2010based differences are observed in neurodevelopmental disorders, and testosterone and estradiol may alter the toxic effect of the compounds. The present study aimed to investigate the toxic effects of methylmercury and thimerosal on SH\u2010SY5Y cells in high testosterone\/low estradiol and high estradiol\/low testosterone containing cellular environment and estimate whether male and female brains react differently to the toxic effects of methylmercury and thimerosal. Study groups (<jats:italic>n<\/jats:italic>\u2009=\u20093) were designed as control: growth medium, thimerosal (T): 1.15\u2010\u03bcM thimerosal, methylmercury (M): 2.93\u2010\u03bcM methylmercury, high testosterone\/low estradiol\u2009+\u2009thimerosal (TT): 1\u2010\u03bcM testosterone\u2009+\u20090.75\u2010\u03bcM estradiol\u2009+\u20091.15\u2010\u03bcM thimerosal, high estradiol\/low testosterone\u2009+\u2009thimerosal (ET): 0.1\u2010\u03bcM testosterone\u2009+\u20097.5\u2010\u03bcM estradiol\u2009+\u20091.15\u2010\u03bcM thimerosal, high testosterone\/low estradiol\u2009+\u2009methylmercury (TM): 1\u2010\u03bcM testosterone\u2009+\u20090.75\u2010\u03bcM estradiol\u2009+\u20092.93\u2010\u03bcM methylmercury and high estradiol\/low testosterone\u2009+\u2009methylmercury (EM): 0.1\u2010\u03bcM testosterone\u2009+\u20097.5\u2010\u03bcM estradiol\u2009+\u20092.93\u2010\u03bcM methylmercury. While a significant decrease in glutathione levels was observed in M group, it was not seen in EM group. A significant increase in the protein carbonyl levels was detected in T group. A similar increase was observed in the TM and TT groups in which testosterone was dominant. It was determined that methylmercury, but not thimerosal, caused significant DNA damage and in TT group. The results showed that both thimerosal and methylmercury are toxic on SH\u2010SY5Y cells and toxic effects of methylmercury are more severe than thimerosal. It has been determined that testosterone and estradiol alter the toxic effects of thimerosal and methylmercury.<\/jats:p>","DOI":"10.1002\/jat.4269","type":"journal-article","created":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T09:07:45Z","timestamp":1638868065000},"page":"981-994","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Modification of the toxic effects of methylmercury and thimerosal by testosterone and estradiol in SH\u2010SY5Y neuroblastoma cell line"],"prefix":"10.1002","volume":"42","author":[{"given":"Selinay Ba\u015fak","family":"Erdemli\u2010K\u00f6se","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Toxicology Hacettepe University Faculty of Pharmacy  Ankara Turkey"},{"name":"Faculty of Arts and Sciences, Department of Chemistry Burdur Mehmet Akif Ersoy University  Burdur 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