{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:30:40Z","timestamp":1791901840707,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T00:00:00Z","timestamp":1640822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["SZSM201511007"],"award-info":[{"award-number":["SZSM201511007"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Emerging evidence suggests that selenium plays an essential role in sperm maturation. However, the specific signaling pathway by which selenium exerts effect has not been elucidated. To evaluate the effect of selenium on GPX4\u2010mediated lipid peroxidation and apoptosis in germ cells, selenium deficiency was modeled by culturing GC2\u2010spd cells in serum\u2010free medium. Treatment with 0.5\u2010\u03bcM sodium selenite (NaSe) or 5.0\u2010\u03bcM selenomethionine (SeMet) significantly improved the proliferation rate and GPX4 protein expression after selenium deficiency. Moreover, NaSe and SeMet decreased the MDA content and lipid peroxidation. When adenovirus was used to knockdown the expression of the GPX4 gene (shRNA\u2010\n                    <jats:italic>GPX4<\/jats:italic>\n                    ), the early apoptosis rate of the shRNA\u2010\n                    <jats:italic>GPX4<\/jats:italic>\n                    cells was significantly higher than that of the EGFP cells. Increased expression of\n                    <jats:italic>Caspase3<\/jats:italic>\n                    and\n                    <jats:italic>Bax<\/jats:italic>\n                    , as well as MDA content were observed in the shRNA\u2010GPX4 cells compared with EGFP cells. In further, overexpression of the GPX4 gene (ORF\u2010\n                    <jats:italic>GPX4<\/jats:italic>\n                    ) cells exhibited increased cell proliferation and decreased MDA content. However, there was no significant difference in 12\/15\u2010lox expression both in ORF\u2010\n                    <jats:italic>GPX4<\/jats:italic>\n                    cells and shRNA\u2010GPX4 cells. Conclusively, GPX4 was involved in the regulation of lipid peroxidation and apoptosis in GC2\u2010spd cells. Selenium played a role in promoting cell proliferation by mediating GPX4. The regulation of GPX4 may occur independently of 12\/15\u2010Lox. These findings confirmed the effect of selenium on spermatogenesis and offered a potential target for treating abnormal semen quality in men.\n                  <\/jats:p>","DOI":"10.1002\/jat.4273","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T20:05:45Z","timestamp":1640894745000},"page":"1016-1028","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The effects of selenium on GPX4\u2010mediated lipid peroxidation and apoptosis in germ cells"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0686-307X","authenticated-orcid":false,"given":"Peiyi","family":"Liu","sequence":"first","affiliation":[{"name":"Affiliated Shenzhen Maternity and Child Healthcare Hospital Southern Medical University  Shenzhen China"},{"name":"Shenzhen Center for Disease Control and Prevention  Shenzhen Guangdong 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