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However, the underlying molecular mechanism of mitochondrial damage remains to be elucidated. In this study, exposure of human promyelocytic leukemia cells (HL\u201060) to 1,4\u2010benzoquinone (1,4\u2010BQ; an active metabolite of benzene) increased the intracellular reactive oxygen species levels, decreased the mitochondrial membrane potential, adenosine triphosphate production and mitochondrial DNA (mtDNA) copy number, up\u2010regulated the expression of mitochondrial fission proteins Drp1 and Fis1, and down\u2010regulated the expression of mitochondrial fusion proteins Mfn2 and Opa1. Further study showed that 1,4\u2010BQ mediated mitochondrial fission through activation of the AMP\u2010activated protein kinase\/mitochondrial fission factor\/dynamin\u2010related protein 1 pathway. Additionally, we also examined the role of exosomal secretion in mitochondrial damage under 1,4\u2010BQ treatment. Results showed that 1,4\u2010BQ increased the total protein level and mtDNA content in exosomes. Upon pre\u2010treatment with the mitochondria\u2010targeted antioxidant SS\u201031, there was attenuation of the mitochondrial damage induced by 1,4\u2010BQ, accompanied by a change in the exosome release characteristics, while inhibition of exosomal secretion using GW4869 aggravated the 1,4\u2010BQ\u2010mediated mitochondrial fission. We concluded that exosomal secretion may serve as a self\u2010protective mechanism of cells against 1,4\u2010BQ\u2010induced mitochondria damage and mitochondrial dynamics interference.<\/jats:p>","DOI":"10.1002\/jat.4328","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T02:08:53Z","timestamp":1649210933000},"page":"1618-1627","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Blocking exosomal secretion aggravates 1,4\u2010benzoquinone\u2010induced mitochondrial fission activated by the AMPK\/MFF\/Drp1 pathway in HL\u201060 cells"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5584-226X","authenticated-orcid":false,"given":"Fangfang","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health and Management Wenzhou Medical University  Zhejiang 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