{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:51:24Z","timestamp":1760575884466,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670724","81871096","82071582"],"award-info":[{"award-number":["81670724","81871096","82071582"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polychlorinated biphenyls (PCBs) affect multiple organs, and some of the effects are mediated by interfering with thyroid hormone (TH) signaling that regulates physiological processes in mammals. It remains unclear how PCBs affect skeletal muscle (SM). In our study, wistar rats were injected 2,3\u2032,4,4\u2032,5\u2010pentachlorobiphenyl (PCB118) intraperitoneally at 0, 10, 100, and 1000\u2009\u03bcg\/kg\/day for 13\u2009weeks, and C2C12 myoblasts were treated PCB118 (0, 0.25, 25, and 50\u2009nM) for 24\u2009h or 48\u2009h. We found that myocyte cross\u2010sectional area (MCSA) was reduced, MyHC IIa and MyHC IIb mRNA levels significantly decreased, and muscle strength was weakened in PCB118\u2010exposed rats. TH receptor \u03b1 (TR\u03b1) and iodothyronine deiodinase type 2 (DIO2) were upregulated after PCB118 exposure both in vivo and in vitro. Transmission electron microscopy showed significant mitochondrial abnormalities in PCB118\u2010treated rats, and the expression of mitochondrial regulators such as PTEN\u2010induced kinase 1 (PINK1) and GTPase dynamin\u2010related protein 1 (DRP1) were altered after PCB118 exposure. These results suggest that PCB118 could weaken muscle strength and attenuate fast\u2010twitch fibers and fiber size of SM in rats. TH signaling, mitochondrial dynamics and mitophagy were also disturbed by PCB118, which may contribute to the alternations of SM structure and function.<\/jats:p>","DOI":"10.1002\/jat.4330","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T23:59:17Z","timestamp":1649721557000},"page":"1628-1638","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["2,3\u2032,4,4\u2032,5\u2010Pentachlorobiphenyl attenuated fast\u2010twitch fibers and fiber size of skeletal muscle via disturbing thyroid hormone signaling and mitochondrial dynamics"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0445-9129","authenticated-orcid":false,"given":"Xiaoxia","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Zou","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Qi","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunlu","family":"Sheng","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Lv","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoxian","family":"Ding","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0035-5915","authenticated-orcid":false,"given":"Yu","family":"Duan","sequence":"additional","affiliation":[{"name":"Division of Geriatric Endocrinology The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1215233"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-018-2272-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/joe-16-0611"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.02128"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2019-00088"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp7058"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005631"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0013-9351(03)00016-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2001.9237"},{"issue":"1","key":"e_1_2_8_11_1","first-page":"5","article-title":"Polychlorinated biphenyls: Persistent pollutants with immunological, neurological, and endocrinological consequences","volume":"16","author":"Crinnion W. J.","year":"2011","journal-title":"Alternative Medicine Review"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(19)31138-9"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10226-9"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7793"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-1490"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.1206198"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287399409531828"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2016.11.011"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-020518-114310"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0892-0362(90)90050-m"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408440600845304"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.54.10.720"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-016-6348-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2018.06.017"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2017.03.013"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-012-1072-0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-019-09908-1"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22329"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-013-0422-4"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00253.2004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03662-0"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2011.07.006"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2013.238"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-3738-8"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1515\/reveh-2012-0040"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfv235"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.278.6.R1545"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-019-02458-x"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4330","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.4330","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T14:49:46Z","timestamp":1760539786000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.4330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,21]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/jat.4330"],"URL":"https:\/\/doi.org\/10.1002\/jat.4330","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"type":"print","value":"0260-437X"},{"type":"electronic","value":"1099-1263"}],"subject":[],"published":{"date-parts":[[2022,4,21]]},"assertion":[{"value":"2021-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}