{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:18:16Z","timestamp":1792005496235,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T00:00:00Z","timestamp":1656892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Molybdenum is an essential dietary trace element required for several critical enzyme systems. High intake is associated with toxicity in ruminants and animal studies. The proposed therapeutic use of molybdenum\u2010based drugs poses a potential risk for accumulation through chronic administration of therapeutic doses of this element. The current experiment was designed to study the effect of daily dosing of a molybdenum compound, bis\u2010choline tetrathiomolybdate (TTM), in Sprague Dawley rats using laser ablation inductively coupled plasma time\u2010of\u2010flight mass spectrometry (LA\u2010ICP\u2010ToF\u2010MS) and two dosing levels of TTM for up to 3\u00a0months. To investigate if molybdenum accumulation was associated with tissue toxicity, histopathology, haematology and clinical biochemistry markers of toxicity were incorporated into the study design. There were no behavioural signs of toxicity to the rats, and no clinical or anatomic pathology was associated with treatment. The current data did show a progressive accumulation of molybdenum within the adrenal gland, kidneys, liver, spleen, brain and testes. Although this was not associated with tissue toxicity within the 3\u2010month study design, greater exposure over a longer period of time has the potential for producing adverse pathophysiological cellular function. Tissue toxicity, as a result of local excessive accumulation of molybdenum over time, has clear implications for the therapeutic use of molybdenum in humans and demands sensitive monitoring of tissue molybdenum levels to avoid toxicity. The current study highlights the shortcomings of conventional biomonitoring approaches to detect molybdenum accumulation with the goal of avoiding molybdenum\u2010associated toxicity.<\/jats:p>","DOI":"10.1002\/jat.4358","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T22:05:07Z","timestamp":1655244307000},"page":"1807-1821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Accumulation of molybdenum in major organs following repeated oral administration of bis\u2010choline tetrathiomolybdate in the Sprague Dawley rat"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9933-1181","authenticated-orcid":false,"given":"John R.","family":"Foster","sequence":"first","affiliation":[{"name":"ToxPath Sciences Ltd  Cheshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kharmen","family":"Billimoria","sequence":"additional","affiliation":[{"name":"LGC Limited, National Measurement Laboratory (NML)  Middlesex UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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