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Their worldwide use and persistence in the environment cause consistent exposure for both humans and aquatic animals. Two siloxane congeners, decamethylcyclopentasiloxane (D5; CAS 541\u201002\u20106) and octamethylcyclotetrasiloxane (D4; CAS 556\u201067\u20102), are among the most prevalent, with measurable levels in air, sediment, water, and biological samples. However, few studies have examined the impact of developmental (embryo\/larva) exposure. To address this gap, we performed parallel experiments using wildtype zebrafish (<jats:styled-content style=\"fixed-case\"><jats:italic>Danio rerio<\/jats:italic><\/jats:styled-content>). One set of experiments used laboratory\u2010mixed individual solutions containing either D4, D5, or 2,4,6,8\u2010tetramethylcyclotetrasiloxane (D<jats:sub>4<\/jats:sub><jats:sup>H<\/jats:sup>; CAS 2370\u201088\u20109); the other used environmental water samples containing a mixture of siloxanes, including D4 and D5. These samples were collected from Bladensburg Waterfront Park (BWP) a site along the Anacostia River, Washington, DC. In both experiments, zebrafish (24\u201348\u2009h postfertilization, hpf) were exposed until 7 or 14\u2009days (d)pf. Chronic exposure to D4, D5, or BWP water until 7 dpf caused stress\u2010like behaviors and reduced swim velocities; anatomical differences were noted only in BWP\u2010exposed larvae. At 14 dpf, BWP\u2010treated larvae still showed slower swimming velocities and increased immobility; anatomical differences were no longer evident and thigmotactic behavior was reduced. D4 and D5\u2010exposed larvae did not survive after 10 dpf. Larvae exposed to D<jats:sub>4<\/jats:sub><jats:sup>H<\/jats:sup> showed no decreases in behavior or growth at either age. These results suggest early developmental sensitivity to siloxane exposure and point to the need to consider embryonic\/larval endpoints when assessing aquatic contaminants.<\/jats:p>","DOI":"10.1002\/jat.4369","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T21:14:25Z","timestamp":1658438065000},"page":"1986-2004","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Developmental effects of siloxane exposure in zebrafish: A comparison study using laboratory\u2010mixed and environmental water samples"],"prefix":"10.1002","volume":"42","author":[{"given":"William H.","family":"Jessup","sequence":"first","affiliation":[{"name":"Department of Biology American University  Washington District of Columbia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenna","family":"Wiegand","sequence":"additional","affiliation":[{"name":"Department of Environmental Science American University  Washington District of Columbia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikayla","family":"Delbridge\u2010Perry","sequence":"additional","affiliation":[{"name":"Department of Chemistry American University  Washington District of Columbia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen E.","family":"MacAvoy","sequence":"additional","affiliation":[{"name":"Department of Environmental Science American University  Washington District of Columbia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6516-7299","authenticated-orcid":false,"given":"Victoria P.","family":"Connaughton","sequence":"additional","affiliation":[{"name":"Department of Biology American University  Washington District of Columbia USA"},{"name":"Center for Neuroscience and Behavior American University  Washington District of Columbia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"AWS. 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