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This study aimed to determine the effectiveness of umbelliferone in MGO\u2010induced apoptosis and oxidative stress in endothelial cells. In this study, it has been indicated that umbelliferone inhibited MGO\u2010induced human umbilical vein endothelial cells (HUVECs) cytotoxicity, apoptosis, Bax\/Bcl\u20102 protein ratio, the activity of cleaved\u2010caspase\u20103, and mitochondrial membrane potential loss. Furthermore, we found that umbelliferone inhibited MGO\u2010induced activation of mitogen\u2010activated protein kinases and nuclear factor\u2010\u03baB signaling pathways in HUVECs. In addition, umbelliferone could suppress oxidative stress, as evidenced by decrease of reactive oxygen species and malondialdehyde (MDA) generation, and increase of superoxide dismutase and glutathione peroxidase contents. Moreover, we found that umbelliferone can activate Nrf2\/HO\u20101 signaling. Importantly, silencing of Nrf2 signaling clearly eliminated the anti\u2010oxidative stress of umbelliferone, whereas umbelliferone pretreatment had no effect on Nrf2 overexpressing HUVECs. Altogether, this study suggested that umbelliferone pretreatment has a protective effect on MGO\u2010induced endothelial cell dysfunction through inhibiting apoptosis and oxidative stress.<\/jats:p>","DOI":"10.1002\/jat.4399","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T13:38:58Z","timestamp":1664372338000},"page":"490-499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Umbelliferone protects against methylglyoxal\u2010induced HUVECs dysfunction through suppression of apoptosis and oxidative stress"],"prefix":"10.1002","volume":"43","author":[{"given":"Shunxiao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Baoshan Branch Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai 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