{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T04:52:03Z","timestamp":1774414323593,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903573"],"award-info":[{"award-number":["81903573"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81930096"],"award-info":[{"award-number":["81930096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vinpocetine injection is often used in clinical treatment of acute cardiovascular and cerebrovascular diseases. However, it was reported that vinpocetine injection caused allergic reactions in clinical use; therefore, its safety needs urgent attention. Until now, research on its sensitization is rarely reported. Here, the components contained in three vinpocetine injections were examined. It was found that besides vinpocetine, the synthetic raw material vincamine, the excipients benzyl alcohol and ethyl <jats:italic>p<\/jats:italic>\u2010toluenesulfonate, and the impurities A, B, C, and D, which are excipients specified in the European Pharmacopoeia, were also present in them. Then the Mas\u2010related G\u2010protein\u2010coupled receptor X2 (MRGPRX2)\u2010HEK293 cell membrane chromatography was used to investigate the affinity of them with MRGPRX2 and found that vinpocetine, vincamine, and impurities A, B, C, and D bind to MRGPRX2. Afterwards, these compounds were further used to investigate the local sensitization ability in vivo. The results showed that vinpocetine, vincamine, and impurity C could induce swelling of the paw and decrease body temperature in mice, but only impurity C could cause local skin mast cell degranulation and serum histamine release increase. In vitro, the results also indicated that impurity C could increase intracellular [Ca<jats:sup>2+<\/jats:sup>] in MRGPRX2\u2010HEK293 cells, whereas vinpocetine and vincamine did not. Therefore, the impurity C was the potential anaphylactoid component in vinpocetine injection, which may be one of the reasons for the occurrence of allergic reactions in the clinical use of vinpocetine injection. This work provides evidence on the sensitization of impurity C and also contributes to promoting the clinical safety of vinpocetine injection.<\/jats:p>","DOI":"10.1002\/jat.4402","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T00:39:20Z","timestamp":1665016760000},"page":"508-516","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Screening potential anaphylactoid components in vinpocetine injection using a high expression <scp>Mas<\/scp>\u2010related G\u2010protein\u2010coupled receptor X2 cell membrane chromatography"],"prefix":"10.1002","volume":"43","author":[{"given":"Jiayu","family":"Lu","sequence":"first","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Shaanxi Institute for Food and Drug Control  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2836-2308","authenticated-orcid":false,"given":"Huaizhen","family":"He","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University  Xi'an Shaanxi 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