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However, the effects of BPA exposure on early testicular development have not been thoroughly studied, and the underlying mechanism is yet to be elucidated. In the current study, neonatal male mice were exposed to BPA at 0, 0.1, and 5\u00a0mg\/kg, respectively, by daily subcutaneous injection during postnatal day (PND) 1\u201335 to explore its effects on testicular development at PND 36 (the end of the first round of spermatogenesis). Morphological analyses showed that BPA exposure significantly induced apoptosis of testicular cells (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and reduced the thickness of seminiferous epithelium (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). In addition, BPA exposure significantly decreased the total antioxidant capacity of testes and levels of transcription factor Nrf2 as well as its downstream antioxidant molecules of NQO1 and GPx\u20101 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Furthermore, global m6A modifications of mRNAs were upregulated accompanied by declined m6A demethylase (FTO) in the testes of BPA groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). MeRIP\u2010quantitative real\u2010time polymerase chain reaction (qPCR) demonstrated that BPA exposure markedly increased the m6A modification of <jats:italic>Nrf2<\/jats:italic> mRNA (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). These findings suggest that upregulation of m6A induced by inhibited FTO may be involved in BPA\u2010induced testicular oxidative stress and developmental injury during postnatal development, which provides a new idea to reveal the mechanism underlying BPA interfering with testicular development.<\/jats:p>","DOI":"10.1002\/jat.4417","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T07:13:48Z","timestamp":1669878828000},"page":"694-705","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Bisphenol A exposure induces testicular oxidative damage via FTO\/m6A\/Nrf2 axis during postnatal development in mice"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1653-8908","authenticated-orcid":false,"given":"Yuanchao","family":"Ling","sequence":"first","affiliation":[{"name":"School of Life Science and Technology Weifang Medical University  Weifang 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