{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T23:07:34Z","timestamp":1766012854365,"version":"build-2065373602"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016302","name":"Coalition for Epidemic Preparedness Innovations","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016302","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007921","name":"University of Pittsburgh","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MV\u2010LASV is an investigational measles Schwarz\u2010based vaccine for the prevention of Lassa fever. A repeated\u2010dose toxicity study in cynomolgus macaques was performed to assess the biodistribution and local and systemic toxicological effects. Monkeys received three immunizations of MV\u2010LASV or saline intramuscularly with a 2\u2010week interval. An increase in anti\u2010measles antibodies confirmed the reaction of the immune system to the vaccine backbone. Clinical observations, body weight, body temperature, local tolerance, electrocardiogram parameters, various clinical pathology parameters (hematology, coagulation urinalysis, serum chemistry, and C\u2010reactive protein) were monitored. Gross pathology and histopathology of various tissues were evaluated. MV\u2010LASV induced a mild increase in fibrinogen and C\u2010reactive protein concentrations. This coincided with microscopic inflammation at the injection sites which partially or fully resolved following a 3\u2010week recovery period. Viral RNA was found in secondary lymphoid organs and injection sites and gall bladder. No viral shedding to the environment was observed. Overall, the vaccine was locally and systemically well tolerated, supporting a first\u2010in\u2010human study.<\/jats:p>","DOI":"10.1002\/jat.4421","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T11:20:44Z","timestamp":1670498444000},"page":"719-733","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biodistribution and toxicology evaluation of a recombinant measles Schwarz\u2010based Lassa vaccine in cynomolgus macaques"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0519-1144","authenticated-orcid":false,"given":"Sabrina","family":"Schrauf","sequence":"first","affiliation":[{"name":"Themis Bioscience GmbH, Vienna, Austria, a subsidiary of Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Tomberger","sequence":"additional","affiliation":[{"name":"Themis Bioscience GmbH, Vienna, Austria, a subsidiary of Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sham","family":"Nambulli","sequence":"additional","affiliation":[{"name":"Center for Vaccine Research University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Microbiology and Molecular Genetics University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Paul","family":"Duprex","sequence":"additional","affiliation":[{"name":"Center for Vaccine Research University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Microbiology and Molecular Genetics University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Tschismarov","sequence":"additional","affiliation":[{"name":"Themis Bioscience GmbH, Vienna, Austria, a subsidiary of Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erich","family":"Tauber","sequence":"additional","affiliation":[{"name":"Themis Bioscience GmbH, Vienna, Austria, a subsidiary of Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Ramsauer","sequence":"additional","affiliation":[{"name":"Themis Bioscience GmbH, Vienna, Austria, a subsidiary of Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00570-17"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3314"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir097"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1920.sp001881"},{"volume-title":"Fields virology","year":"2013","author":"Buchmeier M. J.","key":"e_1_2_9_6_1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.21.11546-11554.2003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1990.03450160063030"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31816857fe"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02633-09"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.430"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(72)90268-4"},{"key":"e_1_2_9_13_1","unstructured":"European Medicines Agency. (2000).Note for guidance on repeated dose toxicity studies. (CPMP\/SWP\/1042\/99).https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/guideline-repeated-dose-toxicity-revision-1_en.pdf"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-2533"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-2762"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1091581815598750"},{"key":"e_1_2_9_17_1","first-page":"1","article-title":"Measles. History and basic biology. Introduction","volume":"329","author":"Griffin D. E.","year":"2009","journal-title":"Current Topics in Microbiology and Immunology"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408360490497456"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1016404108"},{"issue":"3","key":"e_1_2_9_20_1","first-page":"315","article-title":"Nonhuman primate models of measles","volume":"46","author":"Kobune F.","year":"1996","journal-title":"Laboratory Animal Science"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-012-0793-4"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00882-07"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaw3163"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abf6348"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.437"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009788"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.12.013"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0264-410x(01)00495-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-017-1486-3"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10639"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.2000290"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.clpt.6100409"},{"key":"e_1_2_9_33_1","first-page":"89","article-title":"Current animal models: cotton rat animal model","volume":"330","author":"Niewiesk S.","year":"2009","journal-title":"Current Topics in Microbiology and Immunology"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.05.006"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20211930"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.6.3882-3887.2003"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)70043-5"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32488-7"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.9.2485-2488.1995"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2019.07.006"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.5.2186-2192.2000"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.es2015.20.20.21134"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.07.008"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1089\/humc.2012.242"},{"key":"e_1_2_9_45_1","unstructured":"World Health Organization. (2005).WHO Guidelines on nonclinical evaluation of vaccines.https:\/\/www.who.int\/publications\/m\/item\/nonclinical-evaluation-of-vaccines-annex-1-trs-no-927"},{"key":"e_1_2_9_46_1","unstructured":"World Health Organization. (2009).Position paper on measles vaccine.http:\/\/www.who.int\/wer\/2009\/wer8435.pdf?ua=1"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4257-z"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1796528"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4421","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.4421","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:50:58Z","timestamp":1760550658000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.4421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,22]]},"references-count":47,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/jat.4421"],"URL":"https:\/\/doi.org\/10.1002\/jat.4421","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"type":"print","value":"0260-437X"},{"type":"electronic","value":"1099-1263"}],"subject":[],"published":{"date-parts":[[2022,12,22]]},"assertion":[{"value":"2022-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}