{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:05:04Z","timestamp":1792670704703,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,12,25]],"date-time":"2022-12-25T00:00:00Z","timestamp":1671926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2021201024"],"award-info":[{"award-number":["H2021201024"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Asiaticoside is a natural triterpene compound derived from <jats:styled-content style=\"fixed-case\"><jats:italic>Centella asiatica<\/jats:italic><\/jats:styled-content>, possessing confirmed cardioprotective property. However, the roles of asiaticoside in regulating oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R)\u2010caused cardiomyocyte dysfunction remain largely obscure. Human cardiomyocyte AC16 cells were stimulated with OGD\/R to mimic myocardial ischemia\/reperfusion injury and treated with asiaticoside. Cytotoxicity was investigated by CCK\u20108 assay and lactate dehydrogenase (LDH) release analysis. Autophagy\u2010 and Wnt\/\u03b2\u2010catenin signaling\u2010related protein levels were measured via western blotting. Asiaticoside (0\u201320\u2009\u03bcM) did not induce cardiomyocyte cytotoxicity. Asiaticoside (20\u2009\u03bcM) mitigated OGD\/R\u2010induced autophagy, cytotoxicity, oxidative stress, and myocardial injury. Rapamycin, an autophagy inductor, reversed the influences of asiaticoside on autophagy, cytotoxicity, oxidative stress, and myocardial injury, whereas 3\u2010methyadanine, an autophagy inhibitor, played an opposite effect. Asiaticoside (20\u2009\u03bcM) attenuated OGD\/R\u2010induced Wnt\/\u03b2\u2010catenin signaling inactivation, which was reversed after transfection with si\u2010\u03b2\u2010catenin. Transfection with si\u2010\u03b2\u2010catenin attenuated the influences of asiaticoside on autophagy, cytotoxicity, oxidative stress, and myocardial injury. In conclusion, asiaticoside protected against OGD\/R\u2010induced cardiomyocyte cytotoxicity, oxidative stress, and myocardial injury via blunting autophagy through activating the Wnt\/\u03b2\u2010catenin signaling, indicating the therapeutic potential of asiaticoside in myocardial ischemia\/reperfusion injury.<\/jats:p>","DOI":"10.1002\/jat.4424","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T01:04:00Z","timestamp":1671152640000},"page":"789-798","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Asiaticoside attenuates oxygen\u2013glucose deprivation\/reoxygenation\u2010caused injury of cardiomyocytes by inhibiting autophagy"],"prefix":"10.1002","volume":"43","author":[{"given":"Mingyan","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Cardiology Affiliated Hospital of Hebei University  Baoding China"},{"name":"Department of Endocrinology Baoding No.1 Central Hospital  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Affiliated Hospital of Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifang","family":"Guo","sequence":"additional","affiliation":[{"name":"Cardiology Division in Geriatric Institute Hebei Provincial People's Hospital  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Cardiology Affiliated Hospital of Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Cardiology Affiliated Hospital of Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiping","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Cardiology Affiliated Hospital of Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,25]]},"reference":[{"issue":"3","key":"e_1_2_10_2_1","first-page":"1952","article-title":"Research on natural products from traditional Chinese medicine in the treatment of myocardial ischemia\u2010reperfusion injury","volume":"14","author":"Bu W.","year":"2022","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1640708"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20170504"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-020-10096-5"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1074248410370327"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-4272-5_15"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17-0471"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.013896"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12284"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-018-9759-z"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2022.11357"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-020-0403-y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.13144"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/1354750X.2021.1903999"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3794"},{"issue":"3","key":"e_1_2_10_17_1","first-page":"1471","article-title":"Thioredoxin\u20102 protects against oxygen\u2010glucose deprivation\/reperfusion injury by inhibiting autophagy and apoptosis in H9c2 cardiomyocytes","volume":"9","author":"Li Y. Y.","year":"2017","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-017-0011-8"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00762-6"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6121407"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2021.750510"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2020.118926"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01323.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-021-00824-x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/10799893.2020.1756325"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2019.106109"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-1008-6138"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2019.1676585"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2019.4071"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2020.105485"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2022.2061302"},{"issue":"9","key":"e_1_2_10_32_1","first-page":"5634","article-title":"Long non\u2010coding RNA FOXD3\u2010AS1 aggravates ischemia\/reperfusion injury of cardiomyocytes through promoting autophagy","volume":"11","author":"Tong G.","year":"2019","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8624318"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCA.0000000000000997"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00301"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2015.11.001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10557-019-06902-z"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.687935"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00551"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.673677"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21733"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10301"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2020.9182"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-21-6667"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.920325"},{"issue":"5","key":"e_1_2_10_46_1","first-page":"4055","article-title":"SYVN1\/GPX5 axis affects ischemia\/reperfusion induced apoptosis of AC16 cells by regulating ROS generation","volume":"13","author":"Zhang J.","year":"2021","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000754"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00925-z"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109464"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.03.046"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.7217"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00367"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4424","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.4424","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T08:21:41Z","timestamp":1760516501000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.4424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,25]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/jat.4424"],"URL":"https:\/\/doi.org\/10.1002\/jat.4424","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,25]]},"assertion":[{"value":"2022-09-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}