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However, little research has been done on its toxicity to vertebrate embryonic development. In this study, we used zebrafish to assess the effects of roxadustat on early embryonic development. Exposure to 14, 28, and 56\u2009\u03bcM roxadustat resulted in abnormal embryonic development in zebrafish embryos, such as shortened body length and early liver developmental deficiency. Roxadustat exposure resulted in liver metabolic imbalance and abnormal liver tissue structure in adult zebrafish. In addition, roxadustat could up\u2010regulate oxidative stress, and astaxanthin (AS) could partially rescue liver developmental defects by down\u2010regulation of oxidative stress. After exposure to roxadustat, the Notch signaling is down\u2010regulated, and the use of an activator of Notch signaling can partially rescue hepatotoxicity. Therefore, our research indicates that roxadustat may induce zebrafish hepatotoxicity by down\u2010regulating Notch signaling. This study provides a reference for the clinical use of roxadustat.<\/jats:p>","DOI":"10.1002\/jat.4444","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T00:04:18Z","timestamp":1675901058000},"page":"1073-1082","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Roxadustat induces hepatotoxicity in zebrafish embryos via inhibiting Notch signaling"],"prefix":"10.1002","volume":"43","author":[{"given":"Dou","family":"Yang","sequence":"first","affiliation":[{"name":"College of Pharmacy Nanchang University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juhua","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, Jiangxi Provincial Maternal and Child Health Hospital Nanchang University  Nanchang 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