{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T08:36:12Z","timestamp":1771230972351,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of\u00a0Hunan Province","doi-asserted-by":"publisher","award":["2020JJ4082"],"award-info":[{"award-number":["2020JJ4082"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Beryllium sulfate (BeSO<jats:sub>4<\/jats:sub>) can result to lung injuries, such as leading to lipid peroxidation and autophagy, and the treatment of beryllium disease has not been well improved. Ferroptosis is a regulated cell death process driven by iron\u2010dependent and lipid peroxidation, while ferritinophagy is a process mediated by nuclear receptor coactivator 4 (NCOA4), combined with ferritin heavy chain 1 (FTH1) degradation and release Fe<jats:sup>2+<\/jats:sup>, which regulated intracellular iron metabolism and ferroptosis. Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) has the effects of antioxidant, antiautophagy, and antiferroptosis. This study aimed to investigate the effect of H<jats:sub>2<\/jats:sub>S on BeSO<jats:sub>4<\/jats:sub>\u2010induced ferroptosis and ferritinophagy in 16HBE cells and the underlying mechanism. In this study, BeSO<jats:sub>4<\/jats:sub>\u2010induced 16HBE cell injury model was established based on cellular level and pretreated with deferoxamine (DFO, a ferroptosis inhibitor), sodium hydrosulfide (NaHS, a H<jats:sub>2<\/jats:sub>S donor), or NCOA4 siRNA and, subsequently, performed to detect the levels of lipid peroxidation and Fe<jats:sup>2+<\/jats:sup> and the biomarkers of ferroptosis and ferritinophagy. More importantly, our research found that DFO, NaHS, or NCOA4 siRNA alleviated BeSO<jats:sub>4<\/jats:sub>\u2010induced ferroptosis and ferritinophagy by decreasing the accumulation of Fe<jats:sup>2+<\/jats:sup> and lipid peroxides. Furthermore, the relationship between ferroptosis, ferritinophagy, H<jats:sub>2<\/jats:sub>S, and beryllium disease is not well defined; therefore, our research is innovative. Overall, our results provided a new theoretical basis for the prevention and treatment of beryllium disease and suggested that the application of H<jats:sub>2<\/jats:sub>S, blocking ferroptosis, and ferritinophagy may be a potential therapeutic direction for the prevention and treatment of beryllium disease.<\/jats:p>","DOI":"10.1002\/jat.4453","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T02:34:14Z","timestamp":1677465254000},"page":"1169-1182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hydrogen sulfide alleviates beryllium sulfate\u2010induced ferroptosis and ferritinophagy in 16HBE cells"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9168-6970","authenticated-orcid":false,"given":"Xiuli","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine, School of public health, Hengyang Medical School 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