{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:48:33Z","timestamp":1760575713778,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Asbestos is a fibrous silicate mineral exhibiting biopersistence and carcinogenic properties and contributes to mesothelioma. Despite the concept of gene\u2010environmental interaction in pathogenesis of mesothelioma, the possible pathophysiological changes of mesothelial cells simultaneously with SET domain containing 2 (SETD2) loss and asbestos exposure remains obscure. Herein, CRISPR\/Cas9\u2010mediated SETD2 knockout Met\u20105A mesothelial cells (Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup>) were established and exposed with crocidolite, an amphibole asbestos. Cell viability of Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup> appeared to dramatically decrease with \u22652.5\u00a0\u03bcg\/cm<jats:sup>2<\/jats:sup> crocidolite exposure as compared with Met\u20105A, although no cytotoxicity and apoptosis changes of Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup> and Met\u20105A was evident with 1.25\u2009\u03bcg\/cm<jats:sup>2<\/jats:sup> crocidolite exposure for 48\u2009h. RNA sequencing uncovered top 50 differentially expressed genes (DEGs) between 1.25\u2009\u03bcg\/cm<jats:sup>2<\/jats:sup> crocidolite exposed Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup> (Cro\u2010Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup>) and 1.25\u2009\u03bcg\/cm<jats:sup>2<\/jats:sup> crocidolite exposed Met\u20105A (Cro\u2010Met\u20105A), and ITGA4, THBS2, MYL7, RAC2, CADM1, and CLDN11 appeared to be the primary DEGs involved with adhesion in gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Cro\u2010Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup> had strong migration but mild adhesion behavior as compared with Cro\u2010Met\u20105A. Additionally, crocidolite tended to increase migration of Met\u20105A<jats:sup>SETD2\u2010KO<\/jats:sup> but inhibited migration of Met\u20105A when compared with their corresponding cells without crocidolite exposure, although no further adhesion property changes was evident for both cells in response to crocidolite. Therefore, crocidolite may affect adhesion\u2010related gene expression and modify adhesion and migration behavior for SETD2\u2010depleted Met\u20105A, which could provide preliminary insight regarding the potential role of SETD2 in the cell behavior of asbestos\u2010related malignant mesothelial cell.<\/jats:p>","DOI":"10.1002\/jat.4493","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T22:40:30Z","timestamp":1683326430000},"page":"1511-1521","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characteristics of transcription profile, adhesion and migration of SETD2\u2010loss Met\u20105A mesothelial cells exposed with crocidolite"],"prefix":"10.1002","volume":"43","author":[{"given":"Dan","family":"Yang","sequence":"first","affiliation":[{"name":"School of Public Heath Hangzhou Medical College  Hangzhou Zhejiang 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