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A modified MEOGRT was used to evaluate multigenerational effects of 2\u2010ethylhexyl 4\u2010hydroxybenzoate (2\u2010EHHB) under flow\u2010through conditions starting with adults (parent generation, F0) through a 3\u2010week reproductive phase of the second generation (F2). Fish were exposed to one of five 2\u2010EHHB test concentrations or a dechlorinated tap water control. Fecundity was affected at the lowest exposure (5.32\u2009\u03bcg\/L) and greater sensitivity occurred in the F1 and F2 generations. Percent fertility was also diminished from no effect level observed in the F0 generation to 101 and 48.8\u00a0\u03bcg\/L in the F1 and F2 generations, respectively. Growth indices were decreased for F0 adult females and F1 subadults and adults at 48.8\u00a0\u03bcg\/L 2\u2010EHHB. Histopathologic examination of gonads, liver, kidney, and thyroid yielded possible delayed reproductive tract development in F1 subadult males, masculinization of the renal phenotype in F1 adult females (renal tubular eosinophilia) and reduced hepatic energy storage (liver glycogen vacuoles) in F1 (11.3 and 48.8\u00a0\u03bcg\/L) and F2 (48.8 and 101\u2009\u03bcg\/L) males and females, respectively. Endocrine\u2010related findings included a decrease in anal fin papillae in F2 adult males at 101\u2009\u03bcg\/L. Results of this study demonstrate effects on growth, development, and reproduction that may be mediated by endocrine (weak estrogenic) and nonendocrine mechanisms. Duration of the MEOGRT should not be routinely extended beyond the OCSPP 890 guideline study design.<\/jats:p>","DOI":"10.1002\/jat.4502","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T01:50:26Z","timestamp":1687312226000},"page":"1645-1666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of multigenerational effects of 2\u2010ethylhexyl 4\u2010hydroxybenzoate in Japanese medaka"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9495-6393","authenticated-orcid":false,"given":"Sharlene","family":"Matten","sequence":"first","affiliation":[{"name":"Office of Chemical Safety and Pollution Prevention United States Environmental Protection Agency  Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn","family":"Fallacara","sequence":"additional","affiliation":[{"name":"Battelle  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alaa","family":"Kamel","sequence":"additional","affiliation":[{"name":"Office of Chemical Safety and Pollution Prevention United States Environmental Protection Agency  Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott G.","family":"Lynn","sequence":"additional","affiliation":[{"name":"Office of Chemical Safety and Pollution Prevention United States Environmental Protection Agency  Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9071-014X","authenticated-orcid":false,"given":"Douglas J.","family":"Fort","sequence":"additional","affiliation":[{"name":"Fort Environmental Laboratories  Stillwater Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey C.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Experimental Pathology Laboratories, Inc  Sterling Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Leak","sequence":"additional","affiliation":[{"name":"Formerly with Eurofins  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haley","family":"von Holst","sequence":"additional","affiliation":[{"name":"Battelle  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronnie Joe","family":"Bever","sequence":"additional","affiliation":[{"name":"Office of Chemical Safety and Pollution Prevention United States Environmental Protection Agency  Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Thomas","sequence":"additional","affiliation":[{"name":"Battelle  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent J.","family":"Brown","sequence":"additional","affiliation":[{"name":"Battelle  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.45.4.469"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2021.152819"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2014.02.007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2004.01.022"},{"key":"e_1_2_8_6_1","unstructured":"Code of Federal Regulations. 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