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Microsampling, in which small amounts of blood are collected, is attracting attention in this context. ICH S3A Q&amp;A focused on microsampling was published in November 2017 to help accelerate the application of microsampling for toxicokinetic assessment. The increased sensitivity of drug measurement apparatuses such as mass spectrometers has made it possible to measure drug concentrations with small amounts of blood samples. In this review, we summarized the reports on toxicological influence of microsampling in rodents (rats and mice) with or without drug administration or recovery period after blood collection and influences that may arise from differences in the blood sampling site or blood sampling volume. We also summarized some perspectives on further implementation of microsampling in toxicology studies. The use of microsampling in regulatory toxicology studies has gradually increased, although at a lower rate than in discovery studies. Since more animals are used in GLP toxicology studies than in discovery studies, the effect of reducing the number of animals by microsampling is expected to be greater in the toxicology studies. This report aims to promote the application of microsampling to nonclinical studies, as it is beneficial for improving animal welfare and can contribute to the 3Rs.<\/jats:p>","DOI":"10.1002\/jat.4523","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T04:47:43Z","timestamp":1691383663000},"page":"118-128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Impact of microsampling on toxicological evaluation in rodent safety studies"],"prefix":"10.1002","volume":"44","author":[{"given":"Kazuaki","family":"Takahashi","sequence":"first","affiliation":[{"name":"LSIM Safety Institute Corporation  Kamisu Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norimichi","family":"Hattori","sequence":"additional","affiliation":[{"name":"Ajinomoto Co., Inc  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Japan Tobacco Inc  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiro","family":"Jinno","sequence":"additional","affiliation":[{"name":"Axcelead Drug Discovery Partners Inc  Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohiko","family":"Ohtsuka","sequence":"additional","affiliation":[{"name":"Seikagaku Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Nakai","sequence":"additional","affiliation":[{"name":"LSIM Safety Institute Corporation  Kamisu Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuka","family":"Takumi","sequence":"additional","affiliation":[{"name":"Ajinomoto Co., Inc  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Shibui","sequence":"additional","affiliation":[{"name":"Ajinomoto Co., Inc  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koki","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Japan Tobacco Inc  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taishi","family":"Shimazaki","sequence":"additional","affiliation":[{"name":"Japan Tobacco Inc  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"National Institute of Health Sciences  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1035-3454","authenticated-orcid":false,"given":"Kosuke","family":"Saito","sequence":"additional","affiliation":[{"name":"National Institute of Health Sciences  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences International University of Health and Welfare  Otawara Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiro","family":"Saito","sequence":"additional","affiliation":[{"name":"National Institute of Health Sciences  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2015.05.022"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2014.01.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2017-0032"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.727"},{"key":"e_1_2_7_6_1","volume-title":"Second report on priorities for Research and Development in farm animal welfare","author":"Farm Animal Welfare Council (FAWC)","year":"1993"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/1806-9282.63.11.923"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.45.599"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.48.1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2019-0018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2007.05.006"},{"key":"e_1_2_7_12_1","unstructured":"ICH S3A. 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