{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T22:40:56Z","timestamp":1760568056362,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>L\u2010tryptophan has been utilized as a feed additive in animal nutrition to improve growth performance, as well as a dietary supplement to alleviate various emotional symptoms in humans. Despite its benefits, concerns regarding its safety arose following the outbreak of eosinophilia\u2010myalgia syndrome (EMS) among individuals who consumed L\u2010tryptophan. The causative material of EMS was determined to be not L\u2010tryptophan itself, but rather L\u2010tryptophan impurities resulting from a specific manufacturing process. To investigate the effect of L\u2010tryptophan and its impurities on humans who consume meat products derived from animals that were fed L\u2010tryptophan and its impurities, an animal study involving broiler chickens was conducted. The animals in test groups were fed diet containing 0.065%\u20130.073% of L\u2010tryptophan for 27\u2009days. This study aimed to observe the occurrence of toxicological or EMS\u2010related symptoms and analyze the residues of L\u2010tryptophan impurities in meat products. The results indicated that there was no evidence of adverse effects associated with the test substance in the investigated parameters. Furthermore, most of the consumed EMS\u2010causing L\u2010tryptophan impurities did not remain in the meat of broiler chickens. Thus, this study demonstrated the safety of L\u2010tryptophan and some of its impurities as a feed additive.<\/jats:p>","DOI":"10.1002\/jat.4531","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T07:59:24Z","timestamp":1693382364000},"page":"184-200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Toxicology and safety study of L\u2010tryptophan and its impurities for use in broiler feed"],"prefix":"10.1002","volume":"44","author":[{"given":"Soo Min","family":"Ko","sequence":"first","affiliation":[{"name":"Department of Medical Science, AMIST, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Eun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medical Science, AMIST, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"},{"name":"Institute of Biotechnology CJ CheilJedang  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Science, AMIST, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da Som","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Medical Science, AMIST, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyeon","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Medical Science, AMIST, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu\u2010yeol","family":"Son","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology CJ CheilJedang  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In Kyung","family":"Heo","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology CJ CheilJedang  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong Uk","family":"Shin","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology CJ CheilJedang  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9272-6153","authenticated-orcid":false,"given":"Yang Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology CJ CheilJedang  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4052-8745","authenticated-orcid":false,"given":"Woo\u2010Chan","family":"Son","sequence":"additional","affiliation":[{"name":"Department of Pathology, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30514"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386454-3.00018-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.13140\/RG.2.2.34589.97768"},{"key":"e_1_2_7_5_1","unstructured":"Aviagen. 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