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It has been proven to have multiple biological functions and exhibits potential therapeutic effects on hypertension, cancer, and Alzheimer's disease. However, the toxicity of narcissin is largely unknown. Here, we revealed that narcissin treatment led to reduced hatchability, increased malformation rate, shorter body length, and slowed blood flow in zebrafish. Furthermore, bradycardia, pericardial edema, increased SV\u2010BA distance, diminished stroke volume, ejection fraction, and ventricular short\u2010axis shortening rate were also found. A large accumulation of ROS, increased apoptotic cells, and histopathological changes were detected in the heart region. Moreover, the gene expression profiles and molecular docking analysis indicated that Nrf2\/HO\u20101 and calcium signaling pathways were involved in narcissin\u2010induced toxicity. In conclusion, here we provide the first evidence that demonstrates narcissin\u2010induced developmental toxicity and cardiotoxicity in zebrafish via Nrf2\/HO\u20101 and calcium signaling pathways for the first time.<\/jats:p>","DOI":"10.1002\/jat.4545","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T02:43:26Z","timestamp":1695005006000},"page":"344-354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Narcissin induces developmental toxicity and cardiotoxicity in zebrafish embryos via Nrf2\/HO\u20101 and calcium signaling pathways"],"prefix":"10.1002","volume":"44","author":[{"given":"Shuo","family":"Gao","sequence":"first","affiliation":[{"name":"School of Pharmacy Hebei University  Baoding China"},{"name":"Biology Institute Qilu University of Technology (Shandong Academy of Sciences)  Jinan 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