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However, IBC has been reported to be hepatotoxic. Current studies on IBC hepatotoxicity are mostly on normal organisms but lack studies on hepatotoxicity in patients. This study established the depigmented zebrafish model by using phenylthiourea (PTU) and investigated the difference in hepatotoxicity between normal and depigmented zebrafish caused by IBC and the underlying mechanism. Morphological, histological, and ultrastructural examination and RT\u2013qPCR verification were used to evaluate the effects of IBC on the livers of zebrafish larvae. IBC significantly decreased liver volume, altered lipid metabolism, and induced pathological and ultrastructural changes in the livers of zebrafish with depigmentation compared with normal zebrafish. The RNA\u2010sequencing and RT\u2013qPCR results showed that the difference in hepatotoxicity between normal and depigmented zebrafish caused by IBC was closely related to the calcium signaling pathway, lipid decomposition and metabolism, and oxidative stress. This work delved into the mechanism of the enhanced IBC\u2010induced hepatotoxicity in depigmented zebrafish and provided a new insight into the hepatotoxicity of IBC.<\/jats:p>","DOI":"10.1002\/jat.4593","type":"journal-article","created":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T04:20:46Z","timestamp":1708748446000},"page":"919-932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The enhanced hepatotoxicity of isobavachalcone in depigmented zebrafish due to calcium signaling dysregulation and lipid metabolism disorder"],"prefix":"10.1002","volume":"44","author":[{"given":"Huiwen","family":"Zhang","sequence":"first","affiliation":[{"name":"Biology Institute Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyue","family":"Zhu","sequence":"additional","affiliation":[{"name":"Biology Institute Qilu University of 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