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However, its specific role in mitigating myocardial injury induced by acute \u03b2\u2010AR overactivation remains unexplored. This study aimed to investigate the effects and underlying mechanisms of BBR pretreatment in a rat model of acute \u03b2\u2010AR overactivation induced by a single dose of the nonselective \u03b2\u2010adrenergic agonist isoprenaline (ISO). Rats were pretreated with saline or BBR (100\u2009mg\/kg\/day) via gavage for 14 consecutive days, followed by a subcutaneous injection of ISO or saline on the 14th day. The findings indicated that BBR pretreatment significantly attenuated myocardial injury in ISO\u2010stimulated rats, as evidenced by reduced pathological inflammatory infiltration, necrosis, and serum markers of myocardial damage. Additionally, BBR decreased oxidative stress and inflammation in the system and heart. Furthermore, BBR pretreatment enhanced myocardial ATP levels, improved mitochondrial dysfunction through increased Drp1 phosphorylation, and augmented myocardial autophagy. In a CoCl<jats:sub>2<\/jats:sub>\u2010induced H9c2 cell hypoxic injury model, BBR pretreatment mitigated cellular injury, apoptosis, and oxidative stress while upregulating Drp1 and autophagy\u2010associated proteins. Mechanistically, BBR pretreatment activated AKT, AMPK, and LKB1 both in vivo and in vitro, implicating the involvement of the AKT and LKB1\/AMPK signaling pathways in its cardioprotective effects. Our study demonstrated the protective effects of BBR against myocardial injury induced by acute \u03b2\u2010AR overactivation in rats, highlighting the potential of BBR as a preventive agent for myocardial injury associated with \u03b2\u2010adrenergic overactivation.<\/jats:p>","DOI":"10.1002\/jat.4659","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T21:54:21Z","timestamp":1720562061000},"page":"1700-1713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Berberine prevents against myocardial injury induced by acute \u03b2\u2010adrenergic overactivation in rats"],"prefix":"10.1002","volume":"44","author":[{"given":"Yalin","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 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